SHELL NUCLEUS ACCUMBENS NEURONS ARE INVOLVED IN CARDIOVASCULAR AND MICTURITION CONTROL.

SHELL NUCLEUS ACCUMBENS NEURONS ARE INVOLVED IN CARDIOVASCULAR AND MICTURITION CONTROL.

• Autor
• Rodrigo Pigozzi de Carvalho
• Resumo

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  Introduction: Rostral regions of the shell Nucleus Accumbens (NAcc) project to the lateral preoptic area (LPA). Angiotensin-(1-7) injected into the LPA evokes a huge increase in intravesical pressure (IP). Aim: To investigate the possible involvement of shell NAcc in the micturition and cardiovascular control. Methods: protocols were approved by CEUA-CUFMABC (09/2020). Adult male Wistar rats (~450 g) with stainless steel guide cannulas implanted bilaterally in the shell NAcc 7 days prior to the experiments were anesthetized and subjected to cannulation of the femoral artery and vein for mean arterial pressure (MAP) and heart rate recordings (HR) and infusion of drugs, respectively. The urinary bladder was cannulated for IP measurement. A miniaturized Doppler flow probe was placed around the left renal arterial for renal blood flow (RBF) recordings. After the baseline MAP, HR, IP and RBF recordings, GABA (50 mM, 1 µL) or L-glutamate (50 mM, 1 µL) or saline (vehicle, 1 µL) injections were made bilaterally into the shell NAcc and the variables were measured for additional 30 min. Data are as mean±SEM and submitted to Student´s t test (P<0.05). Results: Bilateral injections of GABA into the shell NAcc significantly increased IP (168±11% vs. 5±3%, saline) and renal conductance (RC, 124.67±23.51% vs. 5.45±0.90%, saline), whilst a significant fall in MAP (-64±2 mmHg vs. -2±2 mmHg, saline) and HR (-92±14 bpm vs. 1±2 bpm, saline) were observed compared to saline injections. Bilateral injections of L-glutamate into the shell NAcc significantly increased IP (132±18% vs. 5±3%, saline), and MAP (13±3 mmHg vs. -2±2 mmHg, saline), whereas a significant decrease in RC (-7.39±0.58% vs. 5.45±0.90%, saline) and no changes in HR (13±6 bpm vs. 1±2 bpm, saline) were observed. Conclusion: The shell NAcc participates in the neural circuitry involved in micturition control and plays a possible tonic role in the arterial pressure regulation.

   

• Palavras-chave
• Nucleus accumbens; Cardiovascular control; Micturition control; arterial pressure regulation.
• Área Temática
• Apresentações Orais - Sessão II

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