Skeletal muscle oxidative stress induced by doxorubicin: role of vitamin C

Skeletal muscle oxidative stress induced by doxorubicin: role of vitamin C

• Autor
• Antonio Viana do Nascimento Filho
• Co-autores
• Filipe Fernandes Stoyell-Conti , Gauri Akolkar , Victor Hugo Martins de Miranda , Pawan K. Singal , Maria Cláudia Irigoyen , Kátia De Angelis , Danielle da Silva Dias
• Resumo

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  Introduction: Doxorubicin is associated with cardiotoxicity related to oxidative stress (OS), it also induces skeletal muscle atrophy. Vitamin C (VC) has been used as a pharmacological approach against OS. Evaluate the effects of VC on doxorubicin-induced OS in skeletal muscle. Methods: Male Wistar rats divided into 4 groups (C: Control. VC: Vitamin C. D: Doxorubicin and VCD: VC+Dox, n=8-10/group). Dox was administered in six doses of 2.5 mg/kg for 3 weeks. VC was administered daily (50 mg/kg) for six weeks. OS analysis: antioxidant (catalase [CAT], superoxide dismutase [SOD], glutathione peroxidase [GPx] and non-enzymatic antioxidant capacity [FRAP]), pro-oxidant (NADPH oxidase, hydrogen peroxide [H2O2], nitrites) and tissue damage (protein and lipid oxidation [LPO]) in gastrocnemius. Results: Dox reduced the gastrocnemius weight, VC prevented it (C: 1.74±0.03; VC: 1.55±0.18; D: 0.99±0.06 and VCD: 1.23±0.06 g). No differences in CAT, FRAP, NADPH and nitrites. D showed increased concentration of H2O2 compared to C and VC group, but not to VCD (C: 5.33±0.39; VC: 4.30±1.47; D: 8.72±1.58 and VCD: 3.66±0.73 ?M H2O2). SOD was higher in VCD group compared to the others (C: 8.29±0.61; VC: 8.38±0.48; D: 9.94±0.61 and VCD: 10.38±0.28 USOD/mg protein). GPx was higher in VC and VCD compared to C group and lower in D compared to C and VCD groups (C:0.007±0.002; VC: 0.012±0.002; D: 0.009±0.001 and VCD: 0.011±0.001 nmol/mg protein). Protein oxidation was lower in C group compared to the others (C: 3.63± 0.93; VC: 6.98±0.74; D: 5.69±0.49 and VCD: 5.98±0.29 nmol/mg protein). LPO was higher in D compared to C and VC groups, and lower in VCD compared to D group (C: 342±122; VC:364±153; D: 998±131 and VCD: 389±50 cps/mg protein). Conclusion: Vitamin C protects against doxorubicin-induced skeletal muscle atrophy and oxidative stress, suggesting an approach to management cardio functional disorder in patients under doxorubicin treatment.

   

   

• Palavras-chave
• Oxidative Stress, Doxorubicin, Vitamin C, Skeletal Muscle
• Área Temática
• Apresentações Orais - Sessão I

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