Role of P2X7 purinergic receptors in gastric dismotility in spontaneous hypertensive rats

Role of P2X7 purinergic receptors in gastric dismotility in spontaneous hypertensive rats

• Autor
• Alda Cássia Alves da Silva
• Co-autores
• Kelly Beatriz Vieira de Oliveira , Juliana Soares Severo , Brenda Lois Barros dos Santos , Pedro Henrique Moraes Mendes , Vilk Janne Silva Barros , Luiza Carolinda de Sousa , Joaquim Carlos Coêlho de Oliveira Júnior , Olga Maria Castro de Sousa , Antonio Luiz Martins Maia Filho , Moisés Tolentino Bento da Silva
• Resumo

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  Hypertension etiology may be due to vascular changes, microglial activation in the central nervous system, autonomic imbalance, dysfunction in renin-angiotensin-aldosterone system and purinergic system. Purinergic system disfunction can be related to alterations in motility and gastric emptying in Spontaneous Hypertensive Rats (SHR). In this study, we investigated the effect of an acute dosage of Brilliant Blue G (BBG), a P2X7 antagonist, on gastric emptying of SHR. Male SHR and Male Wistar rats weighing between 180-220g, 4 weeks age, were used. All procedures were performed and approved by the Ethics Committee for Animal Use (CEUA) of the Federal University of Piauí, Brazil, (Protocol 627/20). Each group has an n= 8, divided into: Control, SHR, and SHR + BBG. Rats received BBG (50mg/kg, s.c) or BBG (50mg/kg, s.c) + ATP (2mg/kg s.c) 30-min before the sacrifice. The assessment of gastric emptying was performed by evaluating the gastric contents of the animals after ingesting a solid meal. We observed a decrease (p<0.05) in gastric emptying in the SHR group when compared to control group (SHR: 21.88±2.05% vs Control: 37.95± 2.17%). Treatment with BBG was able to significantly reverse (p<0.05) the EG rate of the SHR group (SHR + BBG: 41.66±3.26 vs SHR: 21.88±2.05%). In order to better understand the role of P2X7 receptors in hypertension associated gastric emptying alterations, we also administered adenosine triphosphate (ATP), a stimulator of purinergic system. Treatment with BBG+ATP returns the gastric emptying rate to similar values to SHR group (SHR+BBG+ATP 25.66±3.01 vs. SHR + BBG: 41.66±3.26 vs SHR: 21.88±2.05%). Hypertension reduces gastric emptying rate, possibly modulate by purinergic system, standing out the involvement of P2X7 receptors. BBG administration was able to reverse the gastric emptying rate reduction.

   

• Palavras-chave
• Hypertension, Purinergic receptors, Gastrointestinal.
• Área Temática
• Apresentações Orais - Sessão VIII

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