The importance of caveolae on eNOS activity in aortas from spontaneously hypertensive rats treated with apocynin.

The importance of caveolae on eNOS activity in aortas from spontaneously hypertensive rats treated with apocynin.

• Autor
• Mariana Santana Quirino da Silva
• Co-autores
• Murilo Eduardo Graton , Cristina Antoniali Silva
• Resumo

• Apocynin induces greater production of NO associated to the increased eNOS activity in systemic blood vessels of spontaneously hypertensive rats (SHR). We hypothesized that apocynin change cell signaling involving caveolae and eNOS in aortas from spontaneously hypertensive rats (SHR). The aim of this study was analyzed the role of caveolae on eNOS activity in aortas from SHR treated with apocynin. To test this hypothesis, we evaluated the reactivity to Acetylcholine in aortic rings of SHR treated with apocynin, in the absence or presence of methyl-?-cyclodextrin (DEX, 10 mmol/L), a drug that disrupts the structure of the caveolae. For the treatment, SHR received apocynin (30 mg/kg, p.o.) in drinking water from the 4th to the 10th week of life.  Wistar rats and SHR not treated with apocynin were used as control groups. We analyzed the vasorelaxant responses of aortic rings to ACh in absence or in presence of DEX or L-NAME (100 µmol/L). DEX shifted to the right and reduced the Emax of concentration-response curves to ACh in aortas of normotensive rats. The reactivity of aortas from SHR to ACh is impaired when compared to aortas from normotensive rats. In the presence of L-NAME, the aorta rings did not respond to ACh as the it did in its absence. The aorta reactivity to ACh in apocynin treated SHR was not different from normotensive rat’s aortas. The results obtained so far suggest that apocynin improves cell signaling involving caveolae and eNOS in SHR aorta, despite improving the reactivity to ACh.

• Palavras-chave
• caveolae, eNOS, SHR, apocynin
• Área Temática
• Vídeo-pôster / Sala 4 - Hipertensão

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