Endothelium dysfunction in gestational hypertension in rats induced by the reduced uteroplacental perfusion pressure model

Endothelium dysfunction in gestational hypertension in rats induced by the reduced uteroplacental perfusion pressure model

• Autor
• Maria Luiza Santos da Silva
• Co-autores
• Laisla Zanetoni Martins , Carlos Alan Candido Dias Junior , Serginara David Rodrigues
• Resumo

•  

  Introduction: Preeclampsia is a disease that arises during pregnancy and is characterized by elevated maternal blood pressure accompanied by 24-hour proteinuria after the 20th week of pregnancy. Its pathogenesis is still unclear. With pregnancy, many changes take place in the cardiovascular system to accommodate the increased blood volume. Studies have shown that deficiencies in the production Nitric Oxide lead to hypertensive disorders. The experimental model of gestational hypertension and PE induced by RUPP (Reduced Uterine Perfusion Pressure) has shown great promise for manifesting the main features found in human PE. Methods: On the 14th day of gestation, the rat was anesthetized with isoflurane and, subsequently, a median incision was made for implantation of a 0.203mm silver clip in the lower abdominal aorta and another two 0.100mm (each) silver clips were implanted in the right and left branches of each ovarian artery. Norm-Preg Group: Pregnant rats that received 0.9% saline solution by gavage. HTN-Preg group: pregnant rats submitted to the RUPP model. Thoracic arteries were dissected in 3 to 4 mm segments. The rings placed in Krebs-Henseleit nutrient solution and changes in aortic tension were recorded using isometric force transducers. Results: We compared the relaxation responses to acetylcholine between strips of aortic vessels from pregnant and hypertensive RUPP rats and found that endothelium-dependent vasodilation was significantly attenuated in hypertensive RUPP rats (66.86±9.47 vs. 41.32±16.80g). Increases in the contraction of the ?-adrenergic receptor agonist phenylephrine have been observed in RUPP group (1.12±0.25 vs. 0.63±0.18g). Conclusion: Thus, RUPP-induced hypertension in pregnant rats has many of the characteristics of preeclampsia in women, objective to simulate the main conditions of preeclampsia, being the way to advance studies of new treatments and solutions for investigating the pathophysiology of this condition that affects thousands of women.

• Palavras-chave
• hipertension, preeclampsia, vascular dysfunction
• Área Temática
• Vídeo-pôster / Sala 9 -O sistema cardiovascular sob a influência do período gestacional ou pós-gestacional ou do dimorfismo sexual

Voltar