Avaliação da produção de citocinas por células mononucleares do sangue periférico de pacientes com asma.

Avaliação da produção de citocinas por células mononucleares do sangue periférico de pacientes com asma.

• Autor
• Viviane de Souza Gomes
• Co-autores
• Larissa Trevisan Carvalho , Matheus André Gomes da Silva , Vitória Fazion Novais , Eduardo Vieira Ponte , Ronei Luciano Mamoni
• Resumo

• Evaluation of cytokines production by peripheral blood mononuclear cells from asthma patients.

  Asthma is a chronic inflammatory disease characterized by inflammation of the airways, leading to its obstruction. In general, this pathology can be classified into two main types, based on the immunopathological mechanisms that lead to its development: atopic asthma and non-atopic asthma. Atopic asthma is characterized by a type I hypersensitivity reaction initiated by exposure to allergens that induce the differentiation of Th2 and Th9 lymphocytes (producing IL-4, IL-5, IL-13, and IL-9), IgE production, sensitization and activation of mast cells and subsequent chemoattraction and activation of eosinophils. Nonatopic asthma is usually associated with exposure to lung infections and pollutants that initiate a chronic inflammatory process with the active participation of Th17 and Th22 lymphocytes (IL-17 and IL-22 producers) and the activation and chemoattraction of neutrophils. Therefore, the different cytokines produced by T lymphocytes play a fundamental role in the development of asthma and, hence, a better understanding of their participation in the evolution of the disease can help in the therapy adopted. In this study, we aimed to evaluate the production of cytokines by peripheral blood mononuclear cells (PBMCs) present in the circulation of patients with asthma. PBMCs were isolated from the blood of patients with asthma or from healthy subjects by centrifugation in ficoll-hyphaque gradient. PBMCs were maintained without stimulation or stimulated with LPS (lipopolysaccharide) or PHA (phytohemagglutinin), and the cytokines (IL-17, IL-22, IFNgamma, IL-4 and IL-9) present in the culture supernatants were quantified by ELISA. Our results showed that PBMCs from patients with asthma produce higher amounts of IL-4, IL9, IL-22 and IL-17, and less IFN-gamma after stimulation when compared to control subjects' cells. In conclusion, our results corroborate previous data from the literature and indicate that these cytokines may play an important role in the development of asthma. 

  Keywords: asthma, peripheral blood mononuclear cells, cytokines.

• Palavras-chave
• asma, células mononucleares do sangue periférico, citocinas.
• Área Temática
• Ciência Básica

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