Interleukins can induce many effects in different areas of the central nervous system (CNS), including retina. Interleukin-4 (IL-4) and interkeukin-6 (IL-6) are classically known as anti-inflammatory and pro-inflammatory cytokines, respectively. Previously we showed that either IL-4 or IL-6 intravitreous injection leads to a sprouting in the retinotectal pathway. Modulation on cholinergic or glutamatergic receptors modify the retinotectal map. The aim of this research was to investigate if IL-4 or IL-6 modulate the content of acetylcholine and glutamate receptor subunits in the visual system. The project was approved by the ethics committee of Fluminense Federal University under protocol number 12909. Lister Hooded rats were treated with IL-4 (5U/µL) or IL-6 (50ng/mL) in the right eye at PND10. Control matched-group received PBS (vehicle) injection. At PND11 or PND14, retinas and superior colliculus (SC) were processed for western blot. Our results showed no difference in GluN1 content (PBS n?4, IL-4 n?3, IL-6 n?3) in the retina and SC of different groups. However, ?2 (PBS n=8, IL-4 n=8, IL-6 n=8) and GluN2B (PBS n=3, IL-4 n=3, IL-6 n=3) subunits were increased in both IL-4 and IL-6 treatments and GluN2A subunit (PBS n=5, IL-4 n=4, IL-6 n=4) was decreased in IL-4 treatment in retinal tissue compared to the control animals. In the subcortical target, GluN2B content (PBS n=3, IL-4 n=3, IL-6 n=3) was increased and GluN2A content (PBS n=4, IL-4 n=5, IL-6 n=3) was decreased in IL-4-treated animals. Moreover pGluA1 subunit (PBS n=4, IL-4 n=3, IL-6 n=4) was increased in IL-6-treated animals in the SC compared to PBS treatment. Together, our data provides evidence that acetylcholine and glutamate receptors could be associated to interleukins effects on retinotectal plasticity.
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Ciências e Cognição
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