The present study proposes the development and characterization of a microneedle-based electrochemical biosensor for detecting IFN-?. The system integrates polymer MNs, chosen for their biodegradability, mechanical robustness, and ease of fabrication, with a PPy coating that serves both as a conductive interface and a functional platform for biomolecule immobilization.
The biosensor demonstrated excellent analytical performance for IFN-? detection, with detection limits comparable to those of commercial ELISA assays, while offering additional advantages, including shorter analysis time, reduced reagent consumption, and label-free detection. The integration of a preconcentration step and the use of electrochemical impedance spectroscopy enabled reliable quantification in both buffer and complex synthetic interstitial fluids. Notably, the biosensor design supports an extraction plus offline analysis workflow, decoupling the sensing and readout stages. This configuration minimizes mechanical interference, eliminates the need for continuous skin contact, and simplifies integration with portable analytical devices—highlighting its potential for decentralized diagnostics.
Altogether, these findings establish the Pol/PPy microneedle biosensor as a cost-effective, scalable, and versatile platform for real-time cytokine monitoring, with promising implications for personalized medicine, immunotherapy management, and infectious disease diagnostics
An article was published.
