Introduction: Gastric cancer, the fifth most incident in Brazil, still represents a significant public health challenge. Conventional chemotherapies present adverse effects that limit their efficacy. In this context, the search for new alternatives becomes essential. Essential oils from the Piperaceae family have been gaining prominence due to their immunomodulatory, anti-inflammatory, and antiproliferative properties. Objectives: To evaluate in vitro antineoplastic activity of Piperaceae family essential oils, extracted at different maturation stages, on a diffuse-type gastric adenocarcinoma model. Methods: The ACP02 cell line was cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin (37°C, 5% CO?). Essential oils from Piperaceae (OEPA, OEPVD, and OEPV) were obtained by hydrodistillation using a Clevenger apparatus. The assays included: cell viability analysis by MTT assay with seeding density of 3×10³ cells/well and treatment with the three oils at concentrations ranging from 100 to 1.15 µg/mL for 72 hours, followed by spectrophotometric reading at 570 nm. For cell death pattern evaluation, cells were treated with IC?? and ½IC?? of each oil, differentially stained with fluorophores, and analyzed by fluorescence microscopy to assess percentages of viable, apoptotic, and necrotic cells in the ACP02 lineage. Negative (untreated) and positive controls (1µM Doxorubicin) were included. Data were analyzed by two-way ANOVA followed by Bonferroni's test (p<0.05). Results: The MTT tests showed that the oils had a cytotoxic effect on the ACP02 strain at all stages of maturation. OEPVD and OEPA exhibited the least cytotoxicity in vitro, requiring average inhibitory concentrations (IC50) of 38.41µg/mL and 43.61µg/mL, respectively, to significantly reduce cell viability. OEPV, on the other hand, showed a more significant reduction in viability compared to the diffrent oils, with an IC50 of 14.43µg/mL. It is worth noting that OEPV showed potent activity from the lowest concentration tested (7.2?g/mL), reaching an effect comparable to the positive control (chemotherapy) at 14.4?g/mL (IC??), with a significant reduction in cell viability. The results of the cell death pattern showed that all essential oils from the Piperaceae family (OEPVD, OEPA and OEPV) showed dose-dependent apoptotic activity in ACP02 cells. OEPVD induced a significant increase in apoptosis at both ½IC50(19.2 ?g/mL) and IC?? (38.4 ?g/mL). OEPA showed a similar effect, with greater apoptosis at 21.8 ?g/mL (½IC??), and 43.6 ?g/mL (IC??). In all cases, the response was predominantly apoptotic. It was observed that the levels of cell death by necrosis were not significant in the three types of oils at different concentrations. Conclusion: Piperaceae essential oils, especially OEPV, showed potential anti-tumor effects against gastric cancer, inducing apoptosis with low necrosis, indicating potential for complementary therapies. Further studies are needed to confirm these findings.
It is with great enthusiasm that we present the Annals of the Oncology International Symposium 2025, an event that continues to solidify its significance in the oncology landscape of northern Brazil. Held in Belém, Pará, Oncology 2025 centered around the theme "The cancer control challenge: better knowing it to best facing it," dedicating itself to exploring the latest frontiers in cancer treatment and prevention.
This year, the symposium provided a deep dive into the essential role of knowledge in the fight against cancer, presenting new perspectives and scientific advancements across various areas of oncology. Renowned global experts gathered to share their most recent research and innovative approaches, offering participants a comprehensive view of the challenges faced by healthcare professionals and patients worldwide.
Presentations and discussions during the event focused on critical topics such as the use of new technologies, advancements in personalized therapies, and more effective prevention strategies. Additionally, particular attention was given to the unique challenges faced by the Amazon region, with efforts aimed at developing region-specific solutions to meet local needs.
Beyond being a high-caliber academic event, Oncology 2025 stood out as a moment for integration and professional networking, with the warm hospitality of the city of Belém offering participants a unique experience. This event became a platform for exchanging ideas, where science, culture, and humanity came together in pursuit of a common goal: to improve cancer control both in Brazil and globally.
This collection of abstracts and articles presented during the event reflects the ongoing dedication to research and the development of innovative solutions, highlighting the importance of collaboration and shared knowledge in the fight against cancer.
General Submission Guidelines:
The presenting author, who does not have to be the first author, must be registered for Oncology 2025.
Each abstract may have up to 10 authors, including the main author and co-authors.
Only original, unpublished work will be accepted.
Submissions must be related to oncology. However, project descriptions, work proposals, experience reports, and literature reviews will not be considered.
Clinical case reports are allowed, provided the abstract addresses scientific questions, details clinical observations, and includes primary scientific data.
The abstract must be written in English, but presentations may be given in Portuguese.
Abstracts must be between 300 and 500 words.
Comissão Organizadora
Comissão Científica
See Annals of Oncology 2023 at:
https://www.even3.com.br/anais/oncology-2023-international-symposium/
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