Introduction: Malignant neoplasms currently represent a significant and persistent challenge for global public health systems. Within the diverse spectrum of malignancies, gastric cancer is particularly noteworthy, ranking as the fourth leading cause of cancer-related mortality worldwide and the fifth most frequently diagnosed cancer in Brazil. Notably, mortality rates for this disease are particularly elevated in the Northern region of the country. While conventional therapies have demonstrated efficacy, their clinical utility is limited by the occurrence of adverse effects, including significant toxicity and the rapid emergence of chemoresistance. In this context, natural products are prominent due to their diverse bioactive properties, with piperine, an alkaloid derived from the specie Piper nigrum L., which belongs to be family Piperaceae, being particularly noteworthy for its mechanisms of action, specifically in mitigating adverse effects and enhancing drug bioavailability. Objectives: The present study aimed to investigate the in vitro antineoplastic activity of isolated piperine on models of primary and metastatic gastric cancer Methods: Piperine crystals were isolated from dried seeds of Piper nigrum L. (black pepper) using a heat-independent method. The analysis was conducted through the assessment of cytotoxicity using the MTT assay, a colorimetric test that evaluates mitochondrial function, and the analysis of the cell death patterns through staining with annexin V and propidium iodide, which allows the differentiation, by fluorescence, between viable cells and those undergoing apoptosis or necrosis, in the AGP-01 (malignant ascites), AGP-01 PIWIL1 KO (with the PIWIL1 gene inactivated), ACP-02 (primary diffuse-type cancer), and ACP-03 (primary intestinal-type cancer) cell lines. Results: Based on the MTT assay data, piperine exhibited cytotoxicity against all tested models, resulting in half-maximal inhibitory concentration (IC50) values of 12,06 µg/mL, 44,32 µg/mL, 26,28 µg/mL and 47,10 µg/mL for the AGP-01, AGP-01 PIWIL1 KO, ACP-02 and ACP-03 cell lines, respectively. These results demonstrate that piperine’s IC50 values varied considerably, with AGP-01 being the most sensitive cell line, presenting the lowest IC50 value. Furthermore, piperine exhibited a selectivity index of 3,6, suggesting the presence of specific mechanisms targeting tumor cells. A concentration-dependent reduction in cell viability was observed across all tested cell lines, starting at 12,5 ?g/mL. Despite its cytotoxic activity in all models, piperine was more active against cells with a more aggressive phenotype, which are characteristic of tumors with higher malignancy. Moreover, due to the higher sensitivity observed in AGP-01, the cell death pattern analysis was performed exclusively on this lineage and indicated that cell death occurred predominantly through apoptosis, with significant differences among the tested concentrations in a concentration-dependent manner. Conclusion: The data obtained highlight the potential of piperine as a therapeutic agent, particularly in the treatment of aggressive gastric cancer, which often exhibits high resistance to conventional therapies. These findings underscore the importance of exploring natural products for the development of novel therapeutic approaches against cancer.
It is with great enthusiasm that we present the Annals of the Oncology International Symposium 2025, an event that continues to solidify its significance in the oncology landscape of northern Brazil. Held in Belém, Pará, Oncology 2025 centered around the theme "The cancer control challenge: better knowing it to best facing it," dedicating itself to exploring the latest frontiers in cancer treatment and prevention.
This year, the symposium provided a deep dive into the essential role of knowledge in the fight against cancer, presenting new perspectives and scientific advancements across various areas of oncology. Renowned global experts gathered to share their most recent research and innovative approaches, offering participants a comprehensive view of the challenges faced by healthcare professionals and patients worldwide.
Presentations and discussions during the event focused on critical topics such as the use of new technologies, advancements in personalized therapies, and more effective prevention strategies. Additionally, particular attention was given to the unique challenges faced by the Amazon region, with efforts aimed at developing region-specific solutions to meet local needs.
Beyond being a high-caliber academic event, Oncology 2025 stood out as a moment for integration and professional networking, with the warm hospitality of the city of Belém offering participants a unique experience. This event became a platform for exchanging ideas, where science, culture, and humanity came together in pursuit of a common goal: to improve cancer control both in Brazil and globally.
This collection of abstracts and articles presented during the event reflects the ongoing dedication to research and the development of innovative solutions, highlighting the importance of collaboration and shared knowledge in the fight against cancer.
General Submission Guidelines:
The presenting author, who does not have to be the first author, must be registered for Oncology 2025.
Each abstract may have up to 10 authors, including the main author and co-authors.
Only original, unpublished work will be accepted.
Submissions must be related to oncology. However, project descriptions, work proposals, experience reports, and literature reviews will not be considered.
Clinical case reports are allowed, provided the abstract addresses scientific questions, details clinical observations, and includes primary scientific data.
The abstract must be written in English, but presentations may be given in Portuguese.
Abstracts must be between 300 and 500 words.
Comissão Organizadora
Comissão Científica
See Annals of Oncology 2023 at:
https://www.even3.com.br/anais/oncology-2023-international-symposium/
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