Utilization of the ompA gene as a molecular marker for Chlamydia abortus

Utilization of the ompA gene as a molecular marker for Chlamydia abortus

• Autor
• Ana Carla dos Santos Wanderley
• Co-autores
• Ariana Messias dos Santos , Guilherme Sarmento Leal Rocha , Caio Andrey Bezerra Januário
• Resumo

• This project tackled the viability of the ompA gene as a molecular marker for Chlamydia abortus, an obligate intracellular bacterium with implications in both medical and animal health contexts. A total of 28 samples of the ompA gene were collected from diverse sources, and a phylogenetic analysis was conducted to assess the gene's conservation among different host groups. The findings revealed that the ompA gene is not an effective molecular marker due to its high conservation within the species. Furthermore, sequences deposited in public databases exhibited unsatisfactory quality, negatively impacting the accuracy of analyses. The limited number of samples also restricted the robustness of evolutionary inferences.

  These findings underscore the complexity of Chlamydia abortus genetic diversity and the need to consider multiple factors when selecting a molecular marker. The lack of variation in the ompA gene across host groups limits its utility for tracing evolutionary and epidemiological relationships. However, the study provided valuable insights for future work. By identifying the limitations of the ompA gene as a molecular marker, the research encourages the exploration of more informative and robust alternatives.

  In summary, this project contributed to understanding the genetic diversity of Chlamydia abortus and evaluating the suitability of the ompA gene as a molecular marker. The phylogenetic analysis revealed that the gene cannot provide distinct insights into the bacterium's evolution and epidemiology due to its conservation and limitations of available sequences. These findings highlight the importance of multidisciplinary approaches and careful consideration of molecular marker characteristics when conducting epidemiological and evolutionary studies related to Chlamydia abortus and related bacteria.

• Palavras-chave
• Bioinformatics, Evolution, Epidemiology, Phylogenetics, Bacteria
• Modalidade
• Pôster
• Área Temática
• Genética/Evolução

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