Binding and Stability of ProlylK-olygopetidase by Enantiomeric Pairs of Chiral Silver Nanoparticles

Binding and Stability of ProlylK-olygopetidase by Enantiomeric Pairs of Chiral Silver Nanoparticles

• Autor
• Carin Cristina da Silva Batista
• Co-autores
• Iseli L. Nantes , Marcelo Yudi Icimoto , Vitor Oliveira
• Resumo

• Binding and Stability of ProlylK-olygopetidase by Enantiomeric Pairs of Chiral Silver Nanoparticles

  Carin C. S. Batista¹, Marcelo Y. Icimoto², Victor Oliveira² and Iseli L. Nantes¹

  1.Federal University of ABC

   

  2.Federal University of São Paulo

  Silver nanoparticles have a diversity of applications both in biological and technological areas. More recently, studies conducted in the interface Nano/Bio have demonstrated that the functionalization of nanostructured materials with chiral molecules and nanocrystals with chiral distortion promoted by the growing in a chiral template present specific properties applicable in advanced materials, energy, medicine, and pharmacology. In the present study, silver nanoparticles were synthesized on silver seeds using D- and L-histidine as templates and boron hydrate as a reducing agent. The nanoparticles were characterized by UV-visible spectroscopy and presented surface plasmon resonance (SPR) bands around 415 nm. The nanoparticles presented CD spectra in the region of the SPR band featured as specular images that are consistent with a pair of enantiomeric nanocrystal with chiral distortion. The silver nanoparticles were also characterized by FESEM-EDX evidenced by the presence of histidine corona. The silver nanoparticles were functionalized with prolyl oligopeptidase (POP) which is a proline-specific endopeptidase, expressed in the brain. This enzyme cleaves neuroactive peptides involved in memory, learning, and neurodegeneration. The enzyme POP was expressed with a His-tag to provide competitive binding affinity to silver nanoparticles covered by D- and L-histidine. Besides the biological importance, POP was chosen as a model for silver nanoparticles functionalized with histidine. The enzyme-functionalized silver nanoparticles were characterized regarding the chiral discrimination for binding affinity and thermal stability. Supported by FAPESP 2017/02317-2 and CNPq 2021/313111-9.

• Palavras-chave
• Silver nanoparticles, functionalization, chiral distortion, oligopeptidase
• Modalidade
• Pôster
• Área Temática
• Nanociência e Nanotecnologia