Gold Nanoparticles Stabilized with Cysteine-Diphenylalanine (CFF) Tripeptide for Acetylcholinesterase Enzyme Immobilization on Printed Carbon Electrodes

  • Autor
  • Marcos Roberto de Araújo Silva
  • Co-autores
  • Bárbara Bianca Gerbelli , Wendel Andrade Alves
  • Resumo
  • With advancements in nanoscience, nanomaterials have become increasingly prominent in various applications due to their unique properties, such as magnetic, electronic, and optical characteristics. Gold nanoparticles (AuNPs), known for their excellent optical and conductive properties, are widely used in diverse areas, including cosmetics and electronic components. The Turkevich method, which involves gold reduction by citrate, is a classical technique for producing gold nanoparticles, resulting in spherical particles with an average hydrodynamic radius of about 30 nm. Other methodologies employ strong reducing agents like sodium borohydride to achieve smaller structures with lower polydispersity. A challenge in nanomaterial synthesis is developing more sustainable processes that minimize environmental impact while maintaining the nanoparticles' efficiency and desired properties. This study introduces a new approach for synthesizing AuNPs using an environmentally friendly synthetic route. This method is based on metal reduction by the cysteine-diphenylalanine (CFF) peptide through a gentle hydrothermal process. The CFF peptide acts as a reducing and morphological controlling agent, producing spherical nanoparticles with hydrodynamic radii smaller than 30 nm, as verified by Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM) techniques. The obtained AuNPs were applied in the chemical modification of printed carbon electrode surfaces, aiming to increase the electroactive area and the electronic transfer constant, confirmed through cyclic voltammetry. This work demonstrates an innovative method for synthesizing gold nanoparticles with potential applications in biosensors and other emerging technologies.

     

  • Palavras-chave
  • Gold nanoparticles, peptide, cysteine-diphenylalanine, electrochemistry, electrode modification
  • Modalidade
  • Pôster
  • Área Temática
  • Materiais Funcionais Avançados
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Bem-vindo(a) aos Anais do VII NanoMat, evento organizado pela Pós-graduação em Nanociências e Materiais Avançados da Universidade Federal do ABC (UFABC) com o intuito de reunir e debater trabalhos desenvolvidos por alunos e pós-doutorandos em Materiais e áreas afins.

  • Nanociência e Nanotecnologia
  • Materiais Funcionais Avançados

Comissão Organizadora

Pedro Alves da Silva Autreto
Andre Luiz Martins de Freitas
Aryane Tofanello

Comissão Científica