Ouabain pretreatment increases B lymphocytes in the inguinal lymph node of mice with subcutaneous melanoma and improves animal survival

Ouabain pretreatment increases B lymphocytes in the inguinal lymph node of mice with subcutaneous melanoma and improves animal survival

• Author
• Mayara Cristini Sebastião Silva
• Co-authors
• Mariana Teixeira Santos Figueiredo Salgado , Maria Luisa Arantes Campos , Ricardo Correia da Silva , Natália Haddad , Mariana Amaral do Nascimento , Manoela Salomon Bernardo , Marlon Silva de Almeida Sampaio , Vinicius Ribeiro Cabral , Luciana Souza de Paiva
• Abstract

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  Introduction: Ouabain (OUA) is a steroid that modulate the immune system. Melanoma is a tumor derived from epidermal melanocytes and is the most lethal skin cancer. In this work we investigated the immunomodulatory and antitumor action of ouabain in a murine model of subcutaneous melanoma. Objective: To investigate whether ouabain modulates B and T lymphocytes and whether this modulation promotes an improvement in the immunological response to melanoma. Material and methods: C57Bl/6 mice were injected intraperitoneally for three consecutive days with DMEM medium in the control (CTR) and melanoma (MEL) groups or with 0.56mg/Kg of OUA in the ouabain (OUA) and melanoma plus ouabain (MEL+OUA) groups. On the 4^th day, the MEL and MEL+OUA groups were inoculated subcutaneously into the flank with B16F10 cells. On the 21^st day, all animals were euthanized for organ removal. Cells were labeled with different antibodies and analyzed by flow cytometry. Parametric statistical tests were used, as appropriate. Results: On the 21^st day, ouabain promoted an increase in the absolute number of B cells in the inguinal lymph node in the MEL+OUA group when compared to the other groups. There was a decrease in the weight and volume of the tumor in animals treated with ouabain when compared to those not treated. Ouabain also increased serum levels of total immunoglobulins M and G in animals with melanoma. Prior treatment with ouabain increased the survival of animals with melanoma. Conclusion: The results point to an effect of ouabain in reducing tumor growth, recruiting B lymphocytes to the tumor's draining lymph node. In addition, ouabain increased serum levels of total IgG and IgM immunoglobulins and the survival of treated animals melanoma carriers.

• Keywords
• Ouabain, immunoregulation, B cells, melanoma
• Subject Area
• Other

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