Canine kidney epithelial MDCK cells are sensitive to ouabain because, when incubated with this drug at concentrations of 250 nM or higher, it binds to its receptor, the Na+/K+-ATPase, and induces cell detachment from their neighbors and the substrate, in a process that requires the activation of the Src-MAPK-STAT3 signaling pathway. On the other hand, monkey kidney epithelial Ma104 cells are resistant to ouabain and do not detach when cultured in the same conditions. When both cell lines are co-cultured in media with ouabain, 50% of ouabain-sensitive MDCK cells remain attached, indicating that resistant cells "rescue" a significant proportion of the sensitive cells. Here, we investigated how Ma104 cells resist ouabain and confer ouabain resistance to sensitive MDCK cells in co-culture. In Ma104, ouabain activates the Src-MAPK-STAT3 signaling pathway with different kinetics than on MDCK cells. Ma104 cells express more extensive and abundant focal contacts than MDCK cells. The extracellular matrix of Ma104 cells is more abundant and enriched in laminin-?1 than the one of MDCK cells. When cultured on a laminin-rich extracellular matrix, these cells acquire ouabain resistance. These results indicate that ouabain-resistant cell adhesion depends, in part, on the expression of a laminin-rich extracellular matrix.
Dear Colleagues and Friends,
We are thrilled to extend a warm welcome to the 8th Annual Meeting of the Cardiotonic Steroids and Na Pump, in collaboration with the Brazilian Society of Pharmacology and Experimental Therapeutic Society (SBFTE) and the Brazilian Society for Biochemistry and Molecular Biology (SBBq). The event is scheduled to take place at the Federal University of São João del Rei (UFSJ) from August 20th to 23rd, 2024.
The Na pump meeting stands as one of Brazil's most enduring scientific gatherings. Over the past decade, it has been hosted by various Brazilian institutions, serving as a pivotal platform for scientific discussions that contribute significantly to the advancement of fundamental knowledge in Na, K-ATPase biochemistry, molecular biology, and related fields. Additionally, it serves as a forum for dialogues on scientific education and training, generating consensus that can influence public policies for the betterment of society.
For this year's meeting, the organizing committee has curated an engaging interdisciplinary program, featuring lectures by esteemed foreign scientists. These sessions will delve into the latest advancements and current challenges across a broad spectrum of research topics in Biochemistry and the Molecular Na pump signaling cascade.
We eagerly anticipate your participation in this exciting scientific event and hope to see you soon in Minas Gerais!
Best regards,
Scientific Committee
Comissão Organizadora
Dr Leandro Augusto Barbosa
Dr Vanessa Faria Cortes
Sílvia Ramos Silva
Marina Vieira
Anna Karolina de Oliveira Alfenas Gadelha - Mídias Sociais e Papelaria
Jessica Alves Faria - Coffee Break e Gráfica
Lucas Antônio Lisboa Ribeiro - Apresentação/Cerimonial
Poliana Amorim Santos - Abertura/ Encerramento/ parte recreativa
?Ana Gabriela Finamore dos Santo-Midias Sociais e Suporte Técnico.
Thiago Malverde de Oliveira - Divulgação (SJDR) e suporte
Maurício Gustavo Oliveira - Credenciamento
Ítalo Leonardo Diogo - Contato com a pós-graduação e Controle de Frequência
Julia Lopes Granato - Midias sociais e controle de frequência
Comissão Científica
Dr. Gustavo Blanco – Kansas University Medical Center, USA
Dr. John Hamlyn – University of Maryland, USA
Dr. Rúben Gerardo Contreras Patiño – CINVESTAV, Mexico
Dra. Vanessa Faria Cortes - Federal University of São João del-Rei, Brazil
Dr Carlos Frederico Leite Fontes - Federal University of Rio de Janeiro, Brazil
Dr Cristoforo Scavone - University of Sao Paulo
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