Glutamate transporters form a family of receptors responsible for the transport of glutamate in astrocytes, preventing the excessive accumulation of this neurotransmitter in the synaptic cleft. Various isoforms of these transporters exist, with GLAST/EAAT1 and GLT-1/EAAT2 being the most highly expressed in astrocytes. Previous studies indicate a potential interaction between the FXYD2 protein and these structures. This study aims to evaluate the effect of incubating the FXYD2 subunit in rat brain tissue, with the goal of increasing the translocation of glutamate transporters to the cell membrane. FXYD2 was extracted from Na,K-ATPase (NKA) purified from pig kidneys. Subsequently, rat brain extracts were prepared (n=6) and divided into two paired groups. One aliquot was incubated for one hour with 70 µg of FXYD2, while the other aliquot volume was assessed with a preparation buffer. A membrane preparation was performed after this incubation. Subsequently, a Western Blot was performed for the EAAT1 transporter, along with the ?1, ?2, and ?3 isoforms, and densitometry was analyzed using ImageJ software. The results showed that the ?1 and ?3 subunits did not show significant changes in their quantities in the membrane. However, the ?2 subunit and the EAAT1 transporter showed an increase of 85% and 89%, respectively, in the quantity present in the membrane in samples incubated with FXYD2. Based on these results, a possible protein-protein interaction between FXYD2, EAAT1, and the ?2 subunit is suggested, which may result in increased translocation of EAAT1 to the membrane of the incubated tissue. This increase in translocation leads to greater glutamate reuptake in the synaptic cleft by astrocytes, reducing the excess of this neurotransmitter that can lead to neurotoxicity.
Dear Colleagues and Friends,
We are thrilled to extend a warm welcome to the 8th Annual Meeting of the Cardiotonic Steroids and Na Pump, in collaboration with the Brazilian Society of Pharmacology and Experimental Therapeutic Society (SBFTE) and the Brazilian Society for Biochemistry and Molecular Biology (SBBq). The event is scheduled to take place at the Federal University of São João del Rei (UFSJ) from August 20th to 23rd, 2024.
The Na pump meeting stands as one of Brazil's most enduring scientific gatherings. Over the past decade, it has been hosted by various Brazilian institutions, serving as a pivotal platform for scientific discussions that contribute significantly to the advancement of fundamental knowledge in Na, K-ATPase biochemistry, molecular biology, and related fields. Additionally, it serves as a forum for dialogues on scientific education and training, generating consensus that can influence public policies for the betterment of society.
For this year's meeting, the organizing committee has curated an engaging interdisciplinary program, featuring lectures by esteemed foreign scientists. These sessions will delve into the latest advancements and current challenges across a broad spectrum of research topics in Biochemistry and the Molecular Na pump signaling cascade.
We eagerly anticipate your participation in this exciting scientific event and hope to see you soon in Minas Gerais!
Best regards,
Scientific Committee
Comissão Organizadora
Dr Leandro Augusto Barbosa
Dr Vanessa Faria Cortes
Sílvia Ramos Silva
Marina Vieira
Anna Karolina de Oliveira Alfenas Gadelha - Mídias Sociais e Papelaria
Jessica Alves Faria - Coffee Break e Gráfica
Lucas Antônio Lisboa Ribeiro - Apresentação/Cerimonial
Poliana Amorim Santos - Abertura/ Encerramento/ parte recreativa
?Ana Gabriela Finamore dos Santo-Midias Sociais e Suporte Técnico.
Thiago Malverde de Oliveira - Divulgação (SJDR) e suporte
Maurício Gustavo Oliveira - Credenciamento
Ítalo Leonardo Diogo - Contato com a pós-graduação e Controle de Frequência
Julia Lopes Granato - Midias sociais e controle de frequência
Comissão Científica
Dr. Gustavo Blanco – Kansas University Medical Center, USA
Dr. John Hamlyn – University of Maryland, USA
Dr. Rúben Gerardo Contreras Patiño – CINVESTAV, Mexico
Dra. Vanessa Faria Cortes - Federal University of São João del-Rei, Brazil
Dr Carlos Frederico Leite Fontes - Federal University of Rio de Janeiro, Brazil
Dr Cristoforo Scavone - University of Sao Paulo
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