Effects of the Cardiotonic Steroid BD-15 on Renal Tissue in Male Wistar Rats Treated with Lipopolysaccharides

Effects of the Cardiotonic Steroid BD-15 on Renal Tissue in Male Wistar Rats Treated with Lipopolysaccharides

• Author
• Pedro Henrique Baia Capanema
• Co-authors
• Jéssica Alves Faria , Antonio Pereira Ribeiro Arantes , Lara Gomes Silva , Maria Eduarda Botelho , Kézia Cristina Alves , Carolina Alves de Oliveira Gotelip , Ana Luiza Menezes Barbosa , Gustavo Fernando de Frazão Lima , Sílvia Ramos Silva , Luciana Estefani Drumond de Carvalho , Leandro Augusto de Oliveira Barbosa , Vanessa Faria Cortes , Hérica de lima Santos , José Augusto Ferreira Perez Villar , Grazielle Aparecida Silva Maia , Israel José Pereira Garcia
• Abstract

•  

  An experimental model for nephritis induction involves treatment with lipopolysaccharide (LPS). The use of the cardiotonic steroid BD-15 at low concentrations (100 ?g/kg, ip) can interact with Na,K-ATPase, mediating intracellular signaling pathways in renal tissue to mitigate damage caused by LPS-induced inflammation. This study aimed to investigate the effects of BD-15 (100 ?g/kg) on renal tissue through biochemical analyses. A total of 20 male Wistar rats were divided into four groups (N = 5): (1) Saline solution, (2) LPS (250 µg/kg, ip), (3) BD-15 (100 ?g/kg, ip), and (4) LPS (250 ?g/kg, ip) + BD-15 (100 ?g/kg, ip). Each animal received the respective treatment and, after 2 hours, was euthanized in accordance with ethical committee requirements. The following parameters were analyzed: Na,K-ATPase activity, lipid peroxidation index (TBARS), catalase activity, protein carbonylation index, and reduced glutathione (GSH) activity. No significant differences were observed in Na,K-ATPase activity and lipid peroxidation; however, intergroup percentage variations were noted in other experiments. Tukey's multiple comparison test revealed a 46% increase in catalase activity in the LPS group (2) compared to the saline solution group (1). For protein carbonylation, the same test detected a 67% increase in the LPS + BD-15 group (4) compared to the BD-15 group (3). Regarding GSH quantification, a reduction was observed in all groups compared to the saline solution group (1): approximately 31% in the LPS group (2), 32% in the BD-15 group (3), and 33% in the LPS + BD-15 group (4). In summary, the results indicate that LPS induced an inflammatory process, while the cardiotonic steroid BD-15 did not cause significant impacts on renal tissue. When BD-15 was administered after LPS induction, it reduced catalase enzyme activity compared to the group treated with LPS alone, indicating potential nephroprotection.

• Keywords
• BD-15, LPS, Kidneys, Na, K-ATPase, Reduced Glutathione.
• Subject Area
• Na Pump

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