Introduction: Cardiotonic steroids (CTS) demonstrated effect in increase collagen production and contributing to the wound healing process. Aim: This study evaluates the effect of 21-benzylidene digoxin (21-BD), a semisynthetic compound of digoxin, on fibroblasts and keratinocytes. Methods: 3T3 (fibroblast) and HaCat (keratinocyte) cells were cultured to confluence in medium containing 0.1% fetal bovine serum and treated with 21-BD for 24 to 72 hours. The cells were then subjected to MTT assay, stress fiber evaluation by immunofluorescence, and cell migration assessment. Results: Preliminary data indicate that 21-BD did not induce cytotoxic effects in either cell type, even at 100 µM for 48 hours. In keratinocytes, an increase in cell proliferation was observed after 24 hours of treatment with 21-BD at 10 nM. Morphological assessment showed that 21-BD increases the number of active fibroblasts and the amount of stress fibers, starting at 1 nM. An increase in stress fibers was also observed in keratinocytes at 1 nM. 21-BD did not affect fibroblast migration but enhanced keratinocyte migration at 1 nM and 10 nM. Conclusion: The data suggest a positive effect of 21-BD on the wound healing process. To better understand these effects, we will evaluate collagen production, the presence of lipid droplets, and the expression of adhesion proteins.
Dear Colleagues and Friends,
We are thrilled to extend a warm welcome to the 8th Annual Meeting of the Cardiotonic Steroids and Na Pump, in collaboration with the Brazilian Society of Pharmacology and Experimental Therapeutic Society (SBFTE) and the Brazilian Society for Biochemistry and Molecular Biology (SBBq). The event is scheduled to take place at the Federal University of São João del Rei (UFSJ) from August 20th to 23rd, 2024.
The Na pump meeting stands as one of Brazil's most enduring scientific gatherings. Over the past decade, it has been hosted by various Brazilian institutions, serving as a pivotal platform for scientific discussions that contribute significantly to the advancement of fundamental knowledge in Na, K-ATPase biochemistry, molecular biology, and related fields. Additionally, it serves as a forum for dialogues on scientific education and training, generating consensus that can influence public policies for the betterment of society.
For this year's meeting, the organizing committee has curated an engaging interdisciplinary program, featuring lectures by esteemed foreign scientists. These sessions will delve into the latest advancements and current challenges across a broad spectrum of research topics in Biochemistry and the Molecular Na pump signaling cascade.
We eagerly anticipate your participation in this exciting scientific event and hope to see you soon in Minas Gerais!
Best regards,
Scientific Committee
Comissão Organizadora
Dr Leandro Augusto Barbosa
Dr Vanessa Faria Cortes
Sílvia Ramos Silva
Marina Vieira
Anna Karolina de Oliveira Alfenas Gadelha - Mídias Sociais e Papelaria
Jessica Alves Faria - Coffee Break e Gráfica
Lucas Antônio Lisboa Ribeiro - Apresentação/Cerimonial
Poliana Amorim Santos - Abertura/ Encerramento/ parte recreativa
?Ana Gabriela Finamore dos Santo-Midias Sociais e Suporte Técnico.
Thiago Malverde de Oliveira - Divulgação (SJDR) e suporte
Maurício Gustavo Oliveira - Credenciamento
Ítalo Leonardo Diogo - Contato com a pós-graduação e Controle de Frequência
Julia Lopes Granato - Midias sociais e controle de frequência
Comissão Científica
Dr. Gustavo Blanco – Kansas University Medical Center, USA
Dr. John Hamlyn – University of Maryland, USA
Dr. Rúben Gerardo Contreras Patiño – CINVESTAV, Mexico
Dra. Vanessa Faria Cortes - Federal University of São João del-Rei, Brazil
Dr Carlos Frederico Leite Fontes - Federal University of Rio de Janeiro, Brazil
Dr Cristoforo Scavone - University of Sao Paulo
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