EFFECT OF BENZYLIDENE DIGOXIN 15 (BD-15) ON MOTOR FUNCTION OF PILOCARPINE-INDUCED EPILEPTIC RATS

EFFECT OF BENZYLIDENE DIGOXIN 15 (BD-15) ON MOTOR FUNCTION OF PILOCARPINE-INDUCED EPILEPTIC RATS

• Author
• Julia Lopes Granato
• Co-authors
• Ítalo Leonardo Diogo , Felipe Santos Teixeira , Gabriela Rodrigues Barbosa , Carolina Alves de Oliveira Gotelip , Gustavo Fernando de Frazão Lima , Grazielle Aparecida Silva Maia , José Augusto Ferreira Perez Villar , Leandro Augusto de Oliveira Barbosa , Cristiane Queixa Tilelli , Luciana Estefani Drumond de Carvalho
• Abstract

•  

  Introduction: Epilepsy is a prevalent neurological condition, particularly involving the temporal lobe. Due to its bilateral connections with the temporal lobe, the cerebellum – which has historically been linked to motor control – may contribute to comorbidity. Propagation of epilepsy beyond the temporal lobe has been linked to cerebellar abnormalities, such as atrophy, which can affect how quickly seizures spread. According to our group (LABIC/UFSJ), BD-15 shows promise in influencing neural function. Assessing motor function and coordination through behavioral testing, such as Grid Walking, may reveal how BD-15 impacts motor learning and neuroplasticity, suggesting the potential for positive effects in the treatment of temporal lobe epilepsy. Objectives: To evaluate the effect of the cardiotonic steroid BD-15 on the motor behavior of male Wistar rats in an experimental model of pilocarpine-induced temporal lobe epilepsy. Materials and Methods: Male Wistar rats, weighing approximately 300 grams, were induced to the experimental model of temporal lobe epilepsy through a dose of pilocarpine (300mg/kg). The animals received BD15 (100 µg/kg; i.p.) for 3 days after induction, while the others received 0.9% saline. Rats were divided into groups as follows: CTR (control) (n=3) and CTR+BD-15 (n=3), NSE (without status epilepticus)  (n=2), NSE+BD-15 (n=7), SE (status epilepticus) (n=6) and SE+BD-15 (n=5). Behavioral assessment of the animals was conducted using the grid walking test in the 6th. Six parameters were evaluated: latency time, total steps, total faults, forelimb foot faults, hindlimb foot faults, and the percentage of total faults in relation to total steps. Results:  Preliminary results do not indicate differences between the groups with regard to motor function assessed through the Grid Walking. Conclusions: Regarding the parameters evaluated, neither the BD-15 or epilepsy were able to change them.

• Keywords
• epilepsy, BD-15, motor coordenation
• Subject Area
• Other

Back Download