Possible Cardiovascular Changes Caused by a High-Carbohydrate Diet and The Effect of Minocycline Administration.

Possible Cardiovascular Changes Caused by a High-Carbohydrate Diet and The Effect of Minocycline Administration.

• Autor
• Alessandra Oliveira Silva
• Co-autores
• Nícia Pedreira Soares , Karla Caroline Marques de Oliveira , Carolina Aparecida de Faria Almeida , Marilene Lopes Ângelo , Daniele Cristina de Aguiar , Stêfany Bruno de Assis Cau , Fernanda Borges de Araújo Paula , Larissa Helena Lobo Torres , Carla Speroni Ceron
• Resumo

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  Cardiovascular diseases are associated with obesity. The consumption of a diet rich in sugars favors increases the adipose tissue and release of anti-inflammatory mediators, which can cause damage to cardiovascular function. Therefore, the objective of the study was to assess the damage caused to the heart tissue by a diet rich in refined carbohydrates (HC) in antioxidant enzymes and to verify the effect of treatment with minocycline. Male Balb/C mice (6 and 7 weeks, 30g) were treated for 12 weeks with a standard diet or HC, and from the 10th to the 12th week, minocycline or saline was administered (CEUA-UFMG 065/2017). The animals were then euthanized and the heart was collected for the analysis of catalase, superoxide dismutase (SOD) and glutathione (GSH) levels. For statistical analysis, two-way Anova was used, followed by Sidak’s post-test. Animals exposed to the HC diet showed increased levels of GSH (DS 0.7566±0.3423; DM 0.8149±0.3805; HCS 2.537±0.6938. HCM 1.43 ±0.4324, p <0.05, n= 3-7/group). Minocycline treatment increased catalase levels in these animals (DS 0.0007881±0.0003482; DM 0.0008429±0.0003452; HCS 0.002712±0.001049; HM 0.005954±0.0009445, p<0.05, n= 3-7/group). No differences were observed in SOD levels (DS 0.7667±0.03629; DM 0.8922±0.08673; HCS 0.9103±0.02627, HCM 0.9530±0.07371, p>0.05 n= 6-8/group). The results show a possible depletion of antioxidant enzymes in HC diet, reduced by minocycline treatment.

   

• Palavras-chave
• Diet, Oxidative stress, Antioxidant enzymes.
• Modalidade
• Comunicação oral
• Área Temática
• Estresse oxidativo