COX-dependent modulation of male sex hormones on the renin-angiotensin-aldosterone vascular reactivity

COX-dependent modulation of male sex hormones on the renin-angiotensin-aldosterone vascular reactivity

• Autor
• Costa, AKN
• Co-autores
• Hortelan, MRM , Athayde, PS , Ribeiro Júnior, RF , Stefanon, I
• Resumo

• Male sex hormone (MSH) has been involved in vascular function regulations. This study evaluated the role of COX pathway in the regulation of vascular reactivity modulated by MSH and the renin-angiotensin-aldosterone system (RAAS). Male Wistar rats, (240-260g), were divided into: orchidectomized (OQT; N=77) and control groups (Sham N=67); the Sham group underwent the same surgical treatment, but without removal of the testicles. Both groups were treated during 12 weeks with losartan (LOS) 15mg/kg/day, i.m.; Apocinine (APO) 30 mg/kg in drinking water, and Spironolactone (SPI) 80mg/kg/day per gavage (CEUA-UFES 17/2020). We evaluated the COX pathway modulation on the concentration-response curves to phenylephrine (Phe) in the presence of indomethacin (INDO), a non-selective COX blocker. The statistical analysis used was anova 1 via plus Tukey post-hoc or Bonferroni. The vasoconstrictor response to Phe was equal in both groups. INDO reduced the maximum response to Phe only in the Sham group (Rmax Sham Control: 107.5±8.9 N=9; Sham INDO: 51.6±7.5* N=7; OQT Control: 107.2±11.8 N=11; OQT INDO: 83.4±10.5 % to KCl 75mM, N=8; *p<0.05). However, both Sham and OQT groups treated with APO, showed a reduction of Rmax to Phe, in the presence of INDO. Both groups treated with SPI presented reduction of Rmax to Phe in the presence of INDO (Rmax: Sham-SPI Control: 121.9±8.1 N=10; Sham-SPI INDO: 81.51±9.0* N=11; OQT-SPI Control: 106.2±11.7 N=14; OQT-SPI INDO: 71.2±8.02* % to KCl 75mM, N=13; *p<0.05). Treatment with LOS did not modify Rmax to Phe. The results suggest that male sex hormone modulates vasoconstriction, by a COX-dependent pathway.

• Palavras-chave
• Testosterone, Vascular Reactivity, Cyclooxygenase.
• Modalidade
• Comunicação oral
• Área Temática
• Sistema endócrino e doenças vasculares