Sex-Specific Effect Of Obesogenic High-Fat Diets On Mesenteric Perivascular Adipose Tissue Function

Sex-Specific Effect Of Obesogenic High-Fat Diets On Mesenteric Perivascular Adipose Tissue Function

• Autor
• Jamaira A. Victorio
• Co-autores
• Daniele M. Guizoni , Israelle N. Freitas , Ana Paula Davel
• Resumo

• Background: The local modulation of vascular tone by perivascular adipose tissue (PVAT) is suggested to be impaired in obesity. Although obesity prevalence is higher in women versus men, sex-specific aspects of PVAT function in obesity remain unclear. We hypothesized that obesity differently impact PVAT function in females versus males. Therefore, we sought to evaluate the PVAT function in contraction and relaxing responses of small mesenteric arteries (SMA) of male and female mice on low-fat, high-fat (HF), and high-fat/high sucrose (HF+HS) diets. Methods: Male and female C57Bl6/J mice (2-month-old) fed chow-diet (CD, 9% of cal. from fat), HF (60% of cal. from fat) or HF+HS (45% of cal. from fat, 30% cal. from sucrose) for 3 or 5 months (CEUA 4914-1/2018, 5474-1/2020). Vascular function was studied in SMA without (-) or with (+) attached PVAT by using a wire myograph. Statistics: *P<0.05, Student´s t test. Results: The anticontractile function of PVAT was greater in females than males at 3 months of CD, which was not observed at 3 months of HF or HF+HS. Indeed, 3-month HF+HS and 5-month HF impaired the anticontractile function of PVAT in females only, while anticontractile effect of PVAT in males was not impaired by either HF or HF+HS. The presence of PVAT did not impact acetylcholine-induced relaxation in SMA of male and female mice on CD or HF. However, in SMA of 3-month HF+HS female mice, PVAT reduced acetylcholine-induced relaxation (Rmax: PVAT-=98±0.6 vs. PVAT+=85±5.3*), effect that was observed in males only at 5 months of HF+HS (Rmax: PVAT-=94±1.7 vs. PVAT+=61±8.0*). Conclusion: The findings suggest that obesogenic HF diets impacts early the PVAT modulation of vascular function. 

• Palavras-chave
• Sex differences; obesity; perivascular adipose tissue.
• Modalidade
• Comunicação oral
• Área Temática
• Tecido adiposo perivascular