Low-Protein Diet During Puberty Causes Endothelial Dysfunction In Aorta From Adult Male Rats

Low-Protein Diet During Puberty Causes Endothelial Dysfunction In Aorta From Adult Male Rats

• Autor
• Amanda Cristina de Souza
• Co-autores
• Anna Rebeka Oliveira Ferreira , Camila Borecki Vidigal , Kawane Fabricio Moura , Deborah Gomes da Silva , Paulo Cezar de Freitas Mathias , Kesia Palma-Rigo , Graziela Scalianti Ceravolo
• Resumo

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  It has been described that low-protein diet during early phases of body development favor the development of cardiometabolic disorders. However, the endothelial consequences of low-protein diet during puberty hasn´t been evaluated. The objective of this study was to evaluate in rats whether protein restriction during puberty causes endothelial dysfunction in aorta and mechanisms involved, also some biometrical parameters was evaluated. Male Wistar rats during adolescence (postnatal day 30 to 60) received either regular rodent diet with 23% of protein (CTR group) or low-protein diet with 4% of protein (LP group). At adulthood, the in vitro thoracic aorta reactivity to phenylephrine were evaluated in the presence or absence of endothelium (E+, E-), indomethacin, apocynin and tempol. The maximum response (Rmax) and pD2 (-log of the concentration of the drug that causes 50% of the Rmax, data not shown) were calculated. The body mass and the naso-anal distance (NAL) and tibial size were evaluated. The data analyzed by ANOVA (one or two-ways and Tukey's) or independent T-test; the results expressed as mean ± s.e.m., differences when * p <0.05. (CEUA/UEL 144/2019). The Rmax to phenylephrine of E+ rings of LP (2,49±0,13, n=9) was increased when compared with CTR (1,51±0,087, n=10). Apocynin reduced Rmax to phenylephrine in LP E+ rings (1,69±0,19, n=8) but not in CTR (2,14±0,18, n=7). Indomethacin and tempol did not interfere with the aortic contraction (data not shown). The NAL (CTR: 24,70±0,28, n=10; LP: 23,80±0,19, n=10), tibia size (CTR: 42,10±0,28, n=10; LP: 40,60±0,16, n=10) and body mass (CTR: 435,47±6,54, n=15; LP: 405,10±6,53, n=15) were reduced in LP rats. The pubertal restriction of protein causes endothelial dysfunction in adulthood, which can favor the development of cardiovascular diseases.

   

• Palavras-chave
• malnutrition, vascular reactivity, oxidative stress
• Modalidade
• Pôster
• Área Temática
• Disfunção endotelial