Protein Restriction Alters Morphology and Anticontractile Function of Aortic Perivascular Adipose Tissue

Protein Restriction Alters Morphology and Anticontractile Function of Aortic Perivascular Adipose Tissue

• Autor
• Israelle Netto Freitas
• Co-autores
• Daniele Mendes Guizoni , Jamaira Aparecida Victorio , Everardo Magalhães Carneiro , Ana Paula Davel
• Resumo

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  Malnutrition is a public health problem in developing countries and a risk for vascular complications. Perivascular adipose tissue (PVAT) is a fat depot that covers most of blood vessels and regulates vascular homeostasis. Here, we hypothesized that protein malnutrition impacts PVAT phenotype and function, which may contribute to a predisposition to cardiovascular diseases in malnourished people. For this, C57Bl/6 mice fed a normoprotein diet (14% protein, C) or a protein-restricted diet (6% protein, isocaloric, R) for 90 days (CEUA No. 4533-1/2017). As the morphology and function of PVAT vary according to the vascular bed, we evaluated the phenotype of the thoracic aortic PVAT (A-PVAT, beige phenotype) and small mesenteric arteries PVAT (M-PVAT, white phenotype). Contraction response to phenylephrine was evaluated in thoracic aortic rings with or without attached PVAT. Statistics: *P<0.05, Student’s t test. As results, the area of A-PVAT adipocytes was greater in R compared to C (C=150±12 vs. R=333±58* µm2) while no difference was found in M-PVAT (C=2291±303 vs. R=2898 ± 959 µm2). Both A-PVAT and M-PVAT of R group exhibited a reduced PPAR-? protein expression (A-PVAT: C=1.3 ± 0.3 vs. R=0.5±0.1*; M-PVAT: C=1.1±0.1 vs. R=0.6 ± 0.1*). UCP-1 was not detected in M-PVAT and no differences were found in A-PVAT from R versus C. As expected, A-PVAT exhibited an anticontractile effect in C (LogEC50: C PVAT-=-7.1±0.2 vs. C PVAT+=-5.8±0.4*). However, this anticontractile effect was impaired in aorta from R (LogEC50: R PVAT-=-6.5±0.3 vs. R PVAT+=-6.8±0.2, P>0.05). Taken together, our results indicate that protein restriction results in a phenotypic and functional changes in A-PVAT with impaired anticontractile effect and increased adipocytes area, although M-PVAT morphology was not affected by protein restriction. Therefore, protein malnutrition may impact PVAT function and morphology depend on the vascular bed.

   

• Palavras-chave
• malnutrition, perivascular adipose tissue, aorta and small mesenteric arteries
• Modalidade
• Pôster
• Área Temática
• Tecido adiposo perivascular