Vascular Reactivity Differences Between Right And Left Pulmonary Artery In Congenital Diaphragmatic Hernia Model In Rabbits

Vascular Reactivity Differences Between Right And Left Pulmonary Artery In Congenital Diaphragmatic Hernia Model In Rabbits

• Autor
• Victoria Araujo Oliveira Maia
• Co-autores
• Lourenço Sbragia , Alecsander Fabricio Moreira Bressan , Fernando Silva Carneiro
• Resumo

• Congenital diaphragmatic hernia (CDH) is a disease that causes an embryologic failure in the diaphragm closing. It is related to pulmonary hypoplasia and a pulmonary vascular remodeling that may cause nitric oxide resistant pulmonary hypertension. CDH affects an estimated rate of 1/3000 newborns in the world. The study aimed to determine the difference between right and left pulmonary artery reactivity to phenylephrine and acetylcholine. We hypothesized that there was a difference in the reactivity of right and left congenital diaphragmatic hernia. Right and left pulmonary arteries from New Zealand newborn rabbits without distinction of sex (40-45g) with CDH (n=6-8) and control (n=6-8) were used. On the 25th day of pregnancy, a surgical incision was made on the left side of the fetal diaphragm to induce CDH mechanically. On the 30th day of pregnancy left and right pulmonary arteries of newborn fetuses were collected. Pulmonary arterial rings were submitted to isometric tension analysis, through concentration-response curves to phenylephrine and acetylcholine. Statistical analyses were established by comparing Emáx and pD2 by Student’s T-test or Anova Two Way followed Tukey post-test. CEUA#123/2020. The response to phenylephrine in CDH is decreased in the left pulmonary arteries  (Emáx-control: 125 ± 5.43 vs. CDH: 97.41 ± 4.58; % KCl) and increased in the right pulmonary arteries(Emáx-control:  88.92 ± 7.82 vs. HDC: 127.90 ± 8.15; % KCl) without difference of pD2. There was no difference in the acetylcholine response. We determined a difference between right and left pulmonary artery in the control group that is inverted in the CDH group, which showed right pulmonary artery increased contractility and left pulmonary artery decreased contractility suggesting that the differences between right and left pulmonary artery function during CDH may be involved in a compensatory mechanism.

• Palavras-chave
• congenital diaphragmatic hernia, pulmonary artery, vascular function
• Modalidade
• Comunicação oral
• Área Temática
• Disfunção endotelial