Coronary Function In Type 1 Diabetic Female Rats: Effects Of Pyridoxamine Treatment

Coronary Function In Type 1 Diabetic Female Rats: Effects Of Pyridoxamine Treatment

• Autor
• Andressa Silva Sousa
• Co-autores
• Matheus Pena Passos , Aline Pincerato Jarrete , Olivia Moraes Ruberti , Maria Andréia Delbin
• Resumo

• Introduction: The formation of advanced glycation end products (AGEs) is an important pathway involved in vascular complications of type 1 diabetes mellitus (DM1), leading to a high risk for coronary artery disease, especially in female. Objective: The study evaluated the vascular reactivity of the coronary artery in type 1 diabetic female rats and the effects of combined treatment with insulin and pyridoxamine (AGEs inhibitor). Methodology: This study was approved by the Ethical Committee for Animal Research Use (CEUA 4532/2017). Female Wistar rats (180-200 g) were divided into: control (CTR), DM1 (DM1, Aloxan 180 mg/kg IP), DM1 treated with insulin (DM1+INS, 2U morning+4U night/day) and DM1 treated with insulin and pyridoxamine (DM1+INS+PDX, 180 mg/kg/day gavage). One week after DM1 induction, the DM1+INS and DM1+INS+PDX groups initiated the treatment for three weeks. Concentration-response curves for acetylcholine (ACh), sodium nitroprusside (SNP) and thromboxane A2 analog (U46619) were performed in septal coronary artery. Biochemical parameters were measured. One?way ANOVA followed by a Tukey's test was performed. Results: The body weight was reduced and the blood glucose was increased in DM1 group. In DM1+INS and DM1+INS+PDX groups the body weights were normalized and the blood glucose was reduced. The maximal response (EMAX) to ACh was decreased in DM1 (72±6%) compared with CTR (97±8%), associated with increased levels of N?-carboxymethylisine (CML) and thiobarbituric acid reactive substances (TBARS). Only the combined treatment in DM1+INS+PDX (99±5%) restored the relaxation response, with no changes in DM1+INS (78±8%) group. The superoxide dismutase (SOD) concentrations were increased in the DM1+INS+PDX group, with a reduction in TBARS values. The CML was reduced in DM1+INS and DM1+INS+PDX groups. Neither the EMAX nor potency (pEC50) were modified for SNP and U46619. Conclusion: The combined treatment with insulin and pyridoxamine prevented the coronary dysfunction and circulatory oxidative stress in type 1 diabetic female rats.

   

• Palavras-chave
• Type 1 Diabetes Mellitus, Septal Coronary Artery, Female Rats, Pyridoxamine.
• Modalidade
• Comunicação oral
• Área Temática
• Disfunção endotelial