EVALUATION OF CONTRACTIBLE AND ELECTRICAL EFFECTS OF THE ETHYL ACETATE FRACTION OFS THE LEAVES OF Syzygium cumini (L.) Skeels IN RAT HEART

EVALUATION OF CONTRACTIBLE AND ELECTRICAL EFFECTS OF THE ETHYL ACETATE FRACTION OFS THE LEAVES OF Syzygium cumini (L.) Skeels IN RAT HEART

• Autor
• Karina Oliveira Mota
• Co-autores
• Andreza Melo de Araujo , Vinícius Cisneiros de Oliveira Santos , Carla Maria Lins Vasconcelos , Brancilene Santos Araújo
• Resumo

• Cardiovascular diseases are a main cause of mortality and morbidity worldwide. It is estimated that 17.9 million people died from cardiovascular disease in 20163. Although there are several medications for these diseases, the search for substances, such as phytochemicals, is essential. In this sense, Syzygium cumini (L.) skeels stands out, a plant abundant in tannins, flavonoids, alkaloids, anthocyanidins1 and polyphenolic compounds4 that are effective against heart disease2. Therefore, the aim of this study was to investigate the action of the ethyl acetate fraction (EAF) on the contractile and electrical properties of the heart. For this, the study was approved by the Ethics Committee on Animal Experimentation nº 7227120520 (ID 000262). Adult Wistar rats (200 and 250 g, n = 6) were used, which were euthanized and the heart was isolated and mounted in a Langendorff type aortic perfusion system (10 mL/min, 34 ± 0.1 ºC). The hearts were perfused with Krebs-Hanseleit solution (control), with different concentrations of EAF (0,001; 0,003; 0,01; 0,03 and 0,1 mg/mL) and then reperfused (washout). Left intraventricular pressure (LIP) and electrocardiogram (ECG) were recorded simultaneously to assess the contractile and electrical effects of EAF on the heart, respectively. Data were tabulated using Excel and GraphPad Prism and expressed as mean ± Mean Standard Error. For the statistical decision, the one-way ANOVA test was used followed by the Tukey test. Probability values of p <0,05 were considered statistically significant. It was observed that the EAF decreased the contractile strength of the heart at concentrations of 0,01 (17,3 ± 4.9), 0,03 (17,1 ± 2,0) and 0,1 mg/mL (11, 8 ± 3,4) compared to the control (38,7 ± 3,6 mm/Hg;), without promoting changes in heart rate and electrocardiographic parameters. Thus, further studies are needed to define the FAE's mechanism of action on it’s parameter.

• Palavras-chave
• Syzygium cumini, Electrocardiogram, Contractility.
• Modalidade
• Comunicação oral
• Área Temática
• Produtos naturais