Red LASER Attenuates And Improves Lipopolysaccharide-Induced Endothelial Dysfunction

Red LASER Attenuates And Improves Lipopolysaccharide-Induced Endothelial Dysfunction

• Autor
• De Melo Arthur, G
• Co-autores
• Moraes, LHO , Terroni, B , Rodrigues, GJ
• Resumo

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  Endothelial function plays an important protective activity in the vascular system and nitric oxide (NO) release plays central role in this protection. For this reason, the low bioavailability of NO is characterized as endothelial dysfunction (ED), a great predictor for the development of several diseases. The availability of NO, therefore, is a therapeutic option for ED. Previously we have verified that red laser (660 nm) is able to induces NO release in vascular tissue. Thus, the objective of this work was to test if the red LASER (660 nm) can be used to treating ED. For this, the endothelial function was evaluated by vascular reactivity in male thoracic aorta rings of Rattus norvegicus albinus rats, of 200 g (~90 days) (CEUA: 7936070618). Aortic rings were arranged in a isometric myograph (Mulvany-Halpern-model 610 DMT, Marietta, GA, USA), in Krebs solution, at 37?C, pH 7.4, gasified by 5% CO2 and 95% O2 and divided in the groups: Control, LPS, LASER PT, LASER RT, LASER+LPS PT and LPS+LASER RT. To induce endothelial dysfunction aortic rings were incubed with LPS (50 µg/mL, 60 min), and control with Krebs solution. Laser treatment (5.6 J, three times) was performed before (preventive treatment = PT) or after (reversive treatment = RT) LPS exposition. After exposition, the aortic rings were contracted with phenylephrine (0.1 ?M) and concentration-effect curves for acetylcholine were made. We have compared the maximum relaxation effect (Emax) using One Way ANOVA (non-parametric) and Tukey's post-test with p<0,05. The “LPS” induced endothelium dysfunction (32.43 ±4.17%, n=8) compared to “control” (91.96 ±2.04%, n=11). Laser treatment improved the relaxation endothelium dependent, both preventive (72.91 ±3.10%, n=6) and reversive (66.72 ±4.83%, n=6). These results suggest that red Laser (660 nm) attenuated and improved endothelial function induced by LPS.

   

• Palavras-chave
• Lasertherapy; Endothelial function; LPS
• Modalidade
• Pôster
• Área Temática
• Disfunção endotelial