Progesterone Prevents the Impairment Effects on the Endothelium Caused by Hormonal Deficiency in Ovariectomized Rats

Progesterone Prevents the Impairment Effects on the Endothelium Caused by Hormonal Deficiency in Ovariectomized Rats

• Autor
• Tagana Rosa da Cunha
• Co-autores
• Jéssyca Aparecida Soares Giesen , Wender Nascimento Rouver , Eduardo Damasceno Costa , Marcella Daruge Grando , Virgínia Soares Lemos , Lusiane Maria Bendhack , Roger Lyrio dos Santos
• Resumo

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  Although progesterone (P4) has a beneficial effect on the cardiovascular system, P4 actions on the coronary bed have not yet been fully elucidated. This study evaluated the effect of progesterone treatment on endothelium-dependent coronary vascular reactivity in ovariectomized Wistar rats. The protocols were approved (63/2017 - CEUA/UFES). Eight-week-old adult rats (BW = 182 ± 14g) were divided into: Sham, Ovariectomized (OVX), Ovariectomized and progesterone treated (OVX P4). The OVX P4 group received daily doses of progesterone (2 mg/kg/day). Vascular reactivity was assessed by a modified Langendorff technique. After stabilization (40 min), a bradykinin curve (BK, 0.1 - 1000 nM), followed by inhibitions of vasodilator synthesis was performed.  The intensity of eNOS, Akt, and gp91phox protein expression was quantified by Western blotting (WB). Superoxide anion and hydrogen peroxide production were measured by fluorescence analysis. Data were expressed as mean ± SEM. The differences among groups were analyzed by one and two-way ANOVA, followed by Tukey post hoc (p <0.05). After administration of BK, we observed that endothelium-dependent vasodilation was impaired in the OVX group and potentiated in the OVX P4 group. (Sham, 14.7 ± 1; OVX, 7.3 ± 1; OVX P4, 23.9 ± 3%; n = 8).  The use of L-NAME enhanced relaxation in the OVX P4 group (23.9 ± 2.8% to 35.2 ± 2.6%).  The response after the combined inhibition of NO and prostanoids was similar to that found with the individual inhibition of NO. Finally, after combined inhibition of NO, PGI₂, and EETs, we observed that the vasodilator response was abolished in all groups, showing the participation of EDH. The results of the fluorescence and WB analysis showed that treatment prevented the alteration in oxidative parameters induced by ovariectomy. The data reported here suggest that P4 has a beneficial action on the coronary bed.

   

• Palavras-chave
• Progesterone, Ovariectomized rats, Coronary reactivity.
• Modalidade
• Comunicação oral
• Área Temática
• Sistema endócrino e doenças vasculares