Multidrug Resistant Protein Inhibitor And Phosphodiesterase Inhibitor PotentiatesThe Vasodilator Effect Induced By Photobiomodulation In Isolated Aortic Rings

Multidrug Resistant Protein Inhibitor And Phosphodiesterase Inhibitor PotentiatesThe Vasodilator Effect Induced By Photobiomodulation In Isolated Aortic Rings

• Autor
• Luis Henrique Oliveira de Moraes
• Co-autores
• Gerson Jhonatan Rodrigues
• Resumo

• Previous Works indicates that Red LASER (660 nm) induces vasodilation by Nitric Oxide (NO) dependent mechanism. NO is able to perfuse smooth muscle cells and activate the enzyme Soluble Guanylate Cyclase, the main NO target in smooth muscle cells. The aim is to study pharmacological strategies that potentiate the vasodilation effect induced by Red LASER in normotensives and hypertensives rats (L-NAME model). Our hypothesis is to find out a pharmacological strategy to potentiates the vasodilator effect of Red LASER controlling intracellular levels of cyclic GMP (cGMP), preventing its efflux (as blocking multidrug-resistant proteins with MK-571) or preventing its degradation (as the use of Sildenafil, a phosphodiesterase inhibitor). It was performed a vascular reactivity experiment in isolated aortic rings from normotensives and hypertensives Wistar rats (Rattus novergicus, Male, weight: 180-200 g; age: 9 weeks) with only one Red LASER and Sodium Nitroprusside application. Analysed using One-Way ANOVA followed by a Newman-Keuls post-test. The study was approved by Animal Use Ethics Committee (CEUA) of the Universidade Federal de São Carlos, on the Protocol number: 7936070618. In aortic rings from normotensive rats, even MK-571 (46.20 ± 5.45%, n = 6), as Sildenafil (59.40 ± 1.86%, n = 6) potentiated the vasodilator effect induced by Red LASER, compared to rings with no previous drug incubation (26.45 ± 1.62%, n = 6). In hypertensive rats, there wasn’t an effect’s potentiation when the rings were previously treated with Sildenafil (5.09 ± 2.33%, n = 8). Despite the aortic rings treated with MK-571 (10.02 ± 6.75%, n = 8) had potentiated vasodilator effect induced by Red LASER compared to control rings (6.75 ± 4.90%, n = 7). Our results indicate that vasodilation induced by Red LASER is potentiated in normotensive rats by MK-571 and Sildenafil. However, only MK-571 potentiates Red LASER effect in hypertensive rats.

• Palavras-chave
• Resistance Proteins, Phosphodiesterase, Photobiomodulation
• Modalidade
• Pôster
• Área Temática
• Sinalização celular