“COMPARISON OF THE EFFECT OF DIFFERENT PROGESTERONE ADMINISTRATION PATHWAYS IN OVARIECTOMIZED WISTAR RATS”

“COMPARISON OF THE EFFECT OF DIFFERENT PROGESTERONE ADMINISTRATION PATHWAYS IN OVARIECTOMIZED WISTAR RATS”

• Autor
• André Lucas Silva Borges
• Co-autores
• Tagana Rosa da Cunha , Roger Lyrio dos Santos
• Resumo

• Even though progesterone is studied for its beneficial effects on the cardiovascular system, comparative analysis of its different administration pathways have not been properly carried out yet. In researches, its actions have been analyzed via direct infusion or through treatment. The latter can be performed by procedures such as injection and placement of a silastic tube capsule, with injection being the most commonly used procedure. However, injections do not promote the stabilization of the serum concentration of the hormone and cause stress to the animal due to handling. Meanwhile, the silastic implant has shown in some studies a capacity of promoting a more stable release and does not require daily handling of the animal, being possibly less stressful. Nevertheless, there are still doubts about whether both procedures promote the same results on cardiovascular parameters or not. Our hypothesis is that the procedures will promote the same effects on coronary perfusion pressure (CPP). Thus, this comparative study aims to evaluate the effect of both administration pathways on the CPP of ovariectomized Wistar rats. Eight-week-old female rats will be divided into Sham, Ovariectomized, Ovariectomized treated with progesterone via silastic tube implants, and Ovariectomized treated with progesterone via subcutaneous injections. The treated groups will receive daily doses of progesterone (2 mg/kg/day), thus, the silastic implants will be filled with 6 mg/200g of it. After 15 days of treatment, the animals will be euthanized and 5 mL of the blood will be collected for serological tests and progesterone concentrations will be determined by immunoassay of electrochemiluminescence using an ELISA® enzyme kit. The basal baseline CPP will be determined by a modified Langendorff technique after 40 minutes of stabilization. Data will be expressed as mean ± SEM and the baseline CPP will be analyzed by one-way ANOVA, followed by Tukey post hoc (p <0.05).

• Palavras-chave
• Progesterone, Administration route, Coronary reactivity.
• Modalidade
• Pôster
• Área Temática
• Revisões, projetos, revisões sistemáticas e metanálises em biologia vascular