Sex Differences in Progesterone-Induced Relaxation in Resistance Mesenteric Arteries

Sex Differences in Progesterone-Induced Relaxation in Resistance Mesenteric Arteries

• Autor
• Izabela Moreira Bonfim
• Co-autores
• Jéssyca Aparecida Soares Giesen , Jocimar José Pitol , Roger Lyrio dos Santos
• Resumo

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  Progesterone plays an important role in the physiological processes related to reproductive organs, but it is already known that it can act in other systems, such as the cardiovascular system. However, little is known about its role on different vascular beds, specifically in resistance arteries, that are the most important vessels in regulating flow and blood pressure. Objective: This work proposes to study the action of progesterone acutely, in order to understand its role in resistance mesenteric arteries and the possible sex difference in relaxing response between normotensive males and females. Our hypothesis is that progesterone has a vasodilatory and protective role in the third-order mesenteric arteries of both sexes. Methods: The experimental procedures will be performed on adult Wistar rats of both sexes (10–12 weeks of age). The third-order mesenteric arteries will be isolated, sectioned into 2-mm rings, and mounted on a resistance myograph system. The relaxation response will be characterised using concentration-response curves of the progesterone (1– 50 ?M). The rings will be incubated with N?-nitro- L-arginine methyl ester (L-NAME), indomethacin, catalase, mifepristone for 30 min. The target protein levels will be measured using Western blots. The level reactive oxygen species (ROS), hydrogen peroxide (H2O2) and nitric oxide (NO) levels will be measured using dihydroethidium (DHE), dichlorofluorescein (DCF) staining and 4,5-diaminofluorescein diacetate (DAF-2), respectively. Data analysis will be performed by the GraphPad Prism 6 (GraphPad Software) and all data expressed as mean±SEM. The significance level of P<0.05 will be established. Registration number of the CEUA-Ufes proposal 18/2020.

   

   

• Palavras-chave
• Progesterone, Vascular Reactivity, Reactive species
• Modalidade
• Pôster
• Área Temática
• Sistema endócrino e doenças vasculares