The Role of Tissular Metalloproteinases-2 e 9 and Inflammatory Markers in Human Abdominal Aortic Aneurysm

The Role of Tissular Metalloproteinases-2 e 9 and Inflammatory Markers in Human Abdominal Aortic Aneurysm

• Autor
• Jéssyca Michelon Barbosa
• Co-autores
• Carlos Alexandre Curylofo Corsi , Fabíola Leslie A. C. Mestriner , Carolina D’Avila Mesquita , Ariel Emiliano S. Couto , Lígia Cristina Borges Campos , Maria Cecília Jordani , Edwaldo E Joviliano , Paulo Roberto Barbosa Évora , Christiane Becari
• Resumo

•  

  Introduction: The abdominal aortic aneurysm (AAA) pathophysiological is not completely understood. Therefore, identifying the mechanism involved in growth, progression, or the risk of rupture in humans could bring a novel treatment perspective. Objective: We sought to investigate the inflammatory markers and metalloproteinases (MMPs) 2 and 9 in aorta obtained from AAA patients. Methodology: The human plasma and aorta from AAA group's were obtained during conventional AAA correction surgeries from HCRP-FMRP-USP. The Control group's plasma and aorta were obtained from patients diagnosed with brain death donor organs and without the aneurysmal disease. The inflammatory markers (IL-6, IL-8, TGF?, and TNF?) were measured in plasma and aorta by radioimmunoassay (ELISA). The MMPs 2 and MMPs 9 were measured in plasma and aorta by zymography. This project was approved by CAAE (n. 82879518.6.0000.5440). Results: Clinical and epidemiological data showed that 80% of the patients were male, with a mean age of 67. Among the risk factors, 98% of patients are or were smokers, and the hypertensive rate was found 61% of patients included, while diabetes (10%) in AAA group. The aorta from the AAA group showed a significant increase of IL-6 (p <0.02), IL-8 (p<0.015) when compared with the control group. However, the plasmatic inflammatory mediators did not show statistical difference between AAA and control groups. Furthermore, aorta and plasma from AAA group showed a significant upregulation in MMP-2 (p=0.024 for plasma, and p= 0,017 for aorta) and MMP-9 (p=0.033 for plasma and p= 0,031 for aorta) compared with the control group. Conclusion: Our data showed an increase in tissular inflammatory markers and MMPS in AAA. Therefore, suggesting that the local/tissue response is an important pathway in the pathophysiology of AAA.

   

• Palavras-chave
• abdominal aortic aneurysm, inflammatory mediators, metalloproteinases
• Modalidade
• Pôster
• Área Temática
• Inflamação e doenças vasculares