Role of NOX-4 Derived Reactive Oxygen Species (ROS) in Perivascular Adipose Tissue (PVAT): Analysis in Microvessels from Ovariectomized and Estrogen-treated Hypertensive Rats

Role of NOX-4 Derived Reactive Oxygen Species (ROS) in Perivascular Adipose Tissue (PVAT): Analysis in Microvessels from Ovariectomized and Estrogen-treated Hypertensive Rats

• Autor
• Bruno Cesar Petroski Moraes
• Co-autores
• Tiago Januário da Costa , Paula Rodrigues de Barros , Simone Regina Potje , Allan Carvalho Mendes , Eliana Hirome Akamine , Rita de Cassia Aleixo Tostes Passaglia
• Resumo

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  Introduction: The reduction in the endogenous estrogen concentration contributes to vascular dysfunction mediated by the increase in reactive oxygen species (ROS) derived from NADPH oxidases. However, the effect of the absence of estrogen on the anti-contractile effect of perivascular adipose tissue (PVAT) has not yet been elucidated. Objective: The project aims to evaluate the expression and role of NOX-4 on the PVAT function of mesenteric arteries of spontaneously hypertensive rats (SHR) ovariectomized (OVX) treated or not with estrogen. Methods: The experimental groups established are SHAM: SHR control females in estrophysiological; OVX: SHR ovariectomized females; Estrogen: SHR females ovariectomized and treated with subcutaneous estrogen (0.1 mg/kg) for 15 days (CEUA-FMRP 0083/2019). Vascular reactivity assay was performed for functional analysis and western blot for molecular. Results were expressed as mean±SEM. The method of analysis was One-way ANOVA followed by Tukey. Results: It has been observed that estrogen can induce NOX-4 expression in immortalized endothelial cell culture (HUVECS). In fact, in the mesenteric arteries, the protein content for NOX-4 is reduced in the OVX group compared to that of SHAM, and the treatment with estrogen restored this parameter. In vascular reactivity, it was observed that the anti-contractile effect of PVAT observed in the arteries of SHAM females (AUC PVAT+: 223.5 ± 27.65; PVAT-: 302.6 ± 43.74) is lost in the OVX group (AUC PVAT+: 267.7 ± 24.44; PVAT-: 268.3 ± 34.44), however the treatment with estrogen restored this effect (AUC PVAT+: 150.8 ± 29.58; PVAT-: 248.2 ± 27.69). The non-specific inhibitor of NOX-4 restored the anti-contractile effect of PVAT in the OVX group (Basal PVAT+: 309.8 ± 14.66 PVAT-: 268.3 ± 34.44 / Inhibitor PVAT+: 224.6 ± 27.49 PVAT -: 395.3 ± 14.52). Conclusion: The reduction in serum estrogen concentration inhibits the anti-contractile effect of PVAT in mesenteric arteries by a NOX-4-dependent mechanism.

   

• Palavras-chave
• Vascular Function, Estrogen, Perivascular Adipose Tissue (PVAT)
• Modalidade
• Pôster
• Área Temática
• Tecido adiposo perivascular