Role Of Egg White Hydrolyzate Against Vascular Damage Promoted By Chronic Ethanol Consumption.

Role Of Egg White Hydrolyzate Against Vascular Damage Promoted By Chronic Ethanol Consumption.

• Autor
• Tatiane Inácio cavallini
• Co-autores
• Samia Hassan Husein Kanaan , Dalton Valentim Vassallo , Marta Miguel , Franck Maciel Peçanha , Giulia Alessandra Wiggers
• Resumo

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  Alcohol consumption is a known cardiovascular risk factor related to hypertension and vascular dysfunction. Functional foods are rich sources of peptides with antioxidant and anti-inflammatory properties, such as egg white hydrolyzate, which could be a protective alternative against vascular damage caused by ethanol. We will investigate whether the egg white hydrolysate (EWH) alters the vascular damage caused by consumption of ethanol (Et) similar to the consumption of a dependent human. in rats. 2-month-old male Wistar rats will be divided and treated for 14 days (CEUA 009/2020): I) Control - drinking water ad libidum and by gavage; II) EWH (drinking water and EWH - 1 g/ kg/day by gavage according to Rizzetti et al. 2016; III) Ethanol (Et - 20% ethanol - drinking water and H2O by gavage) and IV) Et + EWH (20% ethanol - drinking water and EWH by gavage). The ethanol animal groups will undergo an adaptation period of 21 days as described by Marchi et al. (2016). To ensure control of blood pressure and initial vascular condition, an assessment of vascular reactivity will be performed on day 21 and at the end of treatment. The consumption of water and feed will be measured every two days and weekly the systolic blood pressure through caudal plethysmography. After the end of treatment, the rats will be anesthetized and euthanized to remove blood, aorta and mesenteric arteries (MRA) for biochemical analyzes of oxidative stress and vascular reactivity parameters respectively. The vessels will be sectioned in rings and mounted in an organ bathing system and the main vascular pathways will be investigated with the incubation of inhibitors (L-NAME; Apocynin; SOD; Alopurinol; Indomethacin; NS398 and TEA). We expect to clarify whether the administration of EWH modifies/protect the harmful vascular effects of ethanol and the mechanisms involved.

   

• Palavras-chave
• Egg White Hydrolysate, Ethanol, vessel.
• Modalidade
• Pôster
• Área Temática
• Revisões, projetos, revisões sistemáticas e metanálises em biologia vascular