FOR911B, a New Nitric Oxide Donor, Induces Relaxation in Isolated Aorta of Rats

FOR911B, a New Nitric Oxide Donor, Induces Relaxation in Isolated Aorta of Rats

• Autor
• Mirelly Cunha da Silva
• Co-autores
• Déborah Victoria Gomes Nascimento , Ellen Mayara Lima Silva , Gabriela Maria da Silva , Allandeiverson Santos de Sousa , Luiz Gonzaga de França Lopes , Thyago Moreira de Queiroz
• Resumo

• Introduction: The nitric oxide (NO) is a molecule which has multiple functions in the cardiovascular system, among them, the vascular tone control is a pivotal action. The search with new NO donors is an alternative for the innovation in the treatment of cardiovascular diseases. In addition, the prospection is warranted by the limitations of NO donors currently used in clinic. The aim of this study was to investigate the vasorelaxant effect of FOR911B in isolated aorta arteries from rats.  Methods: Wistar rats weighing 250-300 g were used. The aortic rings were isolated and used for isometric tension recordings and evaluation of the vascular smooth muscle-relaxant activity. The aortic rings were kept in Krebs-Henseleit solution, at 37 °C, suspended by cotton threads and gassed with a 95% O2 and 5% CO2, under resting tension of 1.5g. Cumulative response-concentration curves induced by FOR911B were produced in phenylephrine pre-contracted aortic rings. All values were expressed as mean ± SEM. For statistical analysis Graph Pad Prism version 8.0 was used and values with p<0.05 were considered statistically significant. All procedures were approved by CEUA/UFPE (Protocol #0066/2019). Results: FOR911B induced concentration-dependent relaxation in aorta of rats (MR = 119.8 ± 11.9% and pD2 = 6.75 ± 0.1, n = 6). After removal of endothelium, the efficacy and potency of the vasorelaxant response were not altered (MR= 113.3 ± 4.5% and pD2 = 6.64 ± 0.14, n = 6), suggesting that the vasodilator effect induced by FOR911B is endothelium-independent. Conclusion: Based on the preliminary results obtained, it is possible to reveal that FOR911B promotes an endothelium-independent relaxation in aortic artery rings. However, more studies using different pharmacological tools are needed to address the mechanism of action involved in this effect. Now we are investigating the role of sGC/GMPc pathway in the effect elicited by FOR911B.

• Palavras-chave
• Nitric Oxide Donors; Hypertension; Vasorelaxing effect
• Modalidade
• Comunicação oral
• Área Temática
• Disfunção endotelial