Analysis of the Association Between CHGA-415 T/C Polymorphism and Raynaud Syndrome in Systemic Lupus Erythematosus

Analysis of the Association Between CHGA-415 T/C Polymorphism and Raynaud Syndrome in Systemic Lupus Erythematosus

• Autor
• Ana Beatriz Castro Gonçalves
• Co-autores
• Caroline Ferreira Fratelli , Lígia Canongia de Abreu Cardoso Duarte , Calliandra Maria de Souza Silva , Carlos Eduardo Lins , Luzitano Brandão Ferreira , Daniel Oliveira Freire , Izabel Cristina Rodrigues da Silva
• Resumo

• Introduction: Raynaud syndrome is characterized by episodic spasms in peripheral vessels, with perfusion changes, that might cause discomfort and pain. Its occurrence may be associated with Systemic Lupus Erythematosus (SLE), befalling 10 to 45% of this population. Chromogranin A, a product of the CHGA gene, intermediates catecholamines' secretion, which acts in blood vessels' constriction. This gene's functional polymorphism decreases its gene expression, leading to changes in perfusion. Objective: This study aims to determine whether there is an association between the presence of the CHGA-415 T/C polymorphism and Raynaud's Syndrome in an SLE population of the Federal District, Brazil. Methodology: This descriptive study analyzed the CHGA-415 T/C polymorphism in 73 female patients diagnosed with SLE and ages ranging from 18 to 76. A PCR strategy was executed on the DNA extracted from their venous blood samples to detect the TT, TC, and CC genotypes. Data analysis was done by a chi-square test (SPSS 25.0 program), considering a 5% significance level. The University Center of Brasília's research ethics committee approved the present study under opinion nº 1,965,528. Results: There was no statistically significant association between the analyzed genotypes and the Raynaud Syndrome variable in SLE patients (p = 0.5; OR = 0.261; CI = 0.154-2.498). Among patients with homozygous dominant genotype (TT), 21.4% had Raynaud Syndrome compared to 30.5% from other genotypes (TC + CC). Conclusion: Despite not finding a genotypic association, this syndrome was noted in research participants. Thus, further analysis, including other CHGA variants and regions, must be examined to increase the comprehension of the evaluated parameter's potential genetic influence.

• Palavras-chave
• Systemic Lupus Erythematosus, Chromogranin A, Genetic polymorphism
• Modalidade
• Pôster
• Área Temática
• Disfunção endotelial