The Deletion of Elastase-2, an Angiotensin-II Forming Enzyme, Prevents the Abdominal Aortic Aneurysm Development in Mice.

The Deletion of Elastase-2, an Angiotensin-II Forming Enzyme, Prevents the Abdominal Aortic Aneurysm Development in Mice.

• Autor
• Fabiola Mestriner
• Co-autores
• Carolina D`Avila Mesquita , Jéssica M Barbosa , Carlos A C Corsi , Ligia Cristina Borges Campos , Ariel Emiliano Souza do Couto , Maria Júlia Garbellini Diab , Maria Cecília Jordani , Vanessa de Souza Nakagi , Edwaldo Edner Joviliano , Paulo Roberto Barbosa Evora , Maurício Serra Ribeiro , Christiane Becari
• Resumo

•  

  Introduction: Elastase-2 (ELA-2) is an enzyme that generates angiotensin-II (AngII) in arteries and contributes to resistance arteries and vascular remodeling in animal models. It has also been shown that continuous infusion of AngII induced abdominal aortic aneurysm (AAA) in mice. Therefore, we sought to investigate the role of ELA-2 in an AAA development. Methods: Male C57bl/6 (WT) and ELA2knockout (CELA-2aTm1Bdr; ELA-2 KO) mice were treated with saline or AngII for twenty-eight days by subcutaneously infusion. Mice were divided into four groups: WT treated with saline (WT+SAL), ELA-2KO treated with saline (ELA-2KO+SAL), WT treated with AngII (WT+AngII), and ELA-2KO treated with Ang II (ELA-2KO+AngII). The vascular ultrasound (US) was done before treatment and after 28 days. The abdominal aorta measurements as maximum aortic diameter, aortic para-renal diameter, aortic infra-renal diameter, and aortic supra-renal diameter were collected. A dilated aorta is a risk factor aortic rupture/aneurysm. Statistical analyses were performed by the Shapiro Wilk Normality test, two-way ANOVA with Sidak’s multiple comparisons test. This study is approved by CEUA (n.131/2019). Results: The most dilated aorta as measured by the maximum aorta diameter was detected in WT+AngII group and was 60% higher than in the WT+Saline (p=0.01). There was no significant difference in the maximum aorta diameter between ELA-KO+SAL vs. ELA-2KO+AngII (p=0.8). The measurement of dilation in aortic para-renal and aortic infra-renal showed that AngII induced a dilation in WT+AngII (p<0.05), while not modified these parameters in ELA-2KO+AngII group. The aortic supra-renal diameter parameters and weight showed no differences between groups. Conclusion: These results suggest that ELA-2KO mice might be less susceptible to develop an aorta dilation. Therefore, ELA-2 is a factor that contributes to the formation and/or development of AAA.

• Palavras-chave
• Elastase-2, Abominal Aortic Aneurysm, Angiotensin II
• Modalidade
• Pôster
• Área Temática
• Sistema endócrino e doenças vasculares