IMPACT OF BRAZIL NUT SUPPLEMENTATION ON OXIDATIVE STRESS AND LEFT VENTRICULAR HYPERTROPHY IN NEPHERECTOMIZED RATS

IMPACT OF BRAZIL NUT SUPPLEMENTATION ON OXIDATIVE STRESS AND LEFT VENTRICULAR HYPERTROPHY IN NEPHERECTOMIZED RATS

• Autor
• Agatha Cristie Menezes
• Co-autores
• Patricia Pereira de Almeida , Michele Lima Brito , Nathalia da Silva Costa , Johnatas Maldonado-Campos , Marina Borges Oliveira , Beatriz Oliveira da Cruz , Raphaela Valente A. Ito , Natasha N. Ferreira , Cecília N. Castañon , Viviane A. N. Degani , Aline D'avila Pereira , Dangelo Carlo Magliano , Milena Barcza Stockler-Pinto
• Resumo

• INTRODUCTION: Chronic kidney disease is a risk factor for cardiovascular disease. Dietary management is recommended. In this context, Brazil nut (BN), a source of selenium and bioactive compounds could reduce oxidative stress. OBJECTIVE:  Investigate the impact of BN supplementation on oxidative stress and left ventricular hypertrophy (LFH) in nephrectomized rats. METHODS: Three months-old male Wistar rats, divided into two groups (n=16/group): Nx, submitted to a 5/6 nephrectomy; Sham which suffered surgical stress. After 5 weeks of follow-up, animals were randomized in four groups for eight weeks (n=8/group): Sham and Nx, control diet; Sham5% and Nx5% control diet added with 5% BN (AIN93M). Systolic blood pressure (SBP) was measured. Creatinine (Cr), urea (Ur), total cholesterol (TC), thiobarbituric acid reactive substances (TBARS) and glutathione peroxidase (GPx) analysis were performed on plasma. Heart relative mass (HRM) and morphometric parameters were determined. Statistical analyses were performed. (Ethics’comittee approval: n°956/2017). RESULTS: Nx group presented higher values of SBP compared to the Sham group (Nx: 168,8±21,1 vs. Sham: 124,0 ±18,0 [mmHg], p=0,0007). Cr and Ur were increased in Nx compared to Sham (Cr: 1,0±0,1 vs. 0,6±0,0 [mg/dL], p=0,0073; Ur: 60,3±10,3 vs. 30,3±3,2 [mg/dL], p=0,0079). TC was increased in Nx compared to Sham and Nx5% was increased compared Sham5% (Nx: 80,0±17,4 vs. Sham: 55,5±6,9 [mg/dL], p=0,0168; Nx5%: 85,2±15,8 vs. Sham5%: 58,2±5,4 [mg/dL], p=0,016, respectively). GPx activity was increased in Nx5% compared to Nx (189,3±150,9 vs. 25,1±24,44 [nmol/mL/min], p=0,0431). There was no significant difference between groups in TBARS concentrations (p=0,8851). HRM was increased in the Nx group compared to the Sham group (265,0±5,0 vs 237,0±5,0 [g], p<0,002). A LVH was found in nephrectomized groups compared to sham groups (Nx: 81,98± 9,62 vs Sham: 73,61±4,43 [um], p=0,03; Nx5%: 82,3 ± 8,4 vs Sh5%: 70,8±7,5, p=0,0002). CONCLUSION: BN was able to increase GPx activity in nephrectomized rats.

• Palavras-chave
• Oxidative stress, Brazil nut, Chronic kidney disease
• Modalidade
• Vídeos
• Área Temática
• Estresse oxidativo