Impact Of Circulating Microparticles Of Patients With Covid-19 On The Vascular Function Of Healthy and Malnourished Mice

Impact Of Circulating Microparticles Of Patients With Covid-19 On The Vascular Function Of Healthy and Malnourished Mice

• Autor
• Geiza Rafaela Bobato
• Co-autores
• Daniele Mendes Guizoni , Ligia M Antunes-Correa , Matheus Lavorenti Rocha , Ana Paula Couto Davel
• Resumo

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  Introduction: COVID-19 has spread rapidly around the world with hypertension and pre-existing heart disease being risk factors. It is known that malnutrition is an important metabolic and cardiovascular risk factor, but whether or not malnutrition would increase the susceptibility to cardiovascular damage associated with COVID-19 is not known. Circulating microparticles (MP) are released by several cell types and have been considered biomarkers of infectious diseases and mediators of  cardiovascular injury. Therefore, we hypothesized that MP signaling is involved in  vascular injury in COVID-19 that is aggravated by malnutrition. Objective: To investigate the effect of MPs isolated from the plasma of patients with COVID-19 in vascular function of control and malnourished mice. Methods: This project will use plasma samples from patients with severe COVID-19 that were hospitalized at Hospital de Clinicas- UNICAMP (CAAE 30227920.9.0000.5404). Plasma MP will be quantified and isolated. Then, post-weaned C57Bl/6 mice will be randomly divided in 2 experimental groups: control group fed normoprotein diet and protein restriction  group fed low protein diet (6% protein, isocaloric). The aorta will be isolated and incubated in situ with the MP. Next, vascular reactivity and protein expression of local factors regulating vascular tone will be analyzed. Results and conclusion: It is expected an increase in circulating MP in severe COVID-19 patients that could be mediators of vascular dysfunction. Since protein malnutrition increases the risk of vascular complications, it is expected that MP will be even more damaging in malnutrition.

• Palavras-chave
• Protein Restriction, COVID-19, Vascular Function, Microparticles.
• Modalidade
• Vídeos
• Área Temática
• COVID-19 e complicações vasculares