Effect of Recombinant Matrix Metalloproteinase Type II (MMP-2) On Aortas of Adult Mice After Systemic Administration

Effect of Recombinant Matrix Metalloproteinase Type II (MMP-2) On Aortas of Adult Mice After Systemic Administration

• Autor
• Stefanne de Cássia Pereira da Silva
• Co-autores
• Pricila Rodrigues Gonçalves , Wictoria Farias Dias , Thayná Matos da Cunha Catete , David Silva da Costa , Marta Chagas Monteiro , Keuri Eleutério Rodrigues , Raquel Fernanda Gerlach , Alejandro Ferraz do Prado
• Resumo

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  Introduction: The increase in MMP-2, an extracellular matrix metalloproteinase, is linked to the pathophysiology of several cardiovascular diseases. In the arteries of aging animals, this effect is associated with increased fibrosis by TGF-?1 cytokine. We hypothesize that treatment with recombinant MMP-2 in mice for four weeks promotes morphological changes, such as increased collagen and elastin in the aortas. Objective:  This study aimed to evaluate the morphological changes in mice's aorta after four weeks of treatment with recombinant MMP-2. Methods: MMP-2 was expressed and purified in E. coli bacteria stem from the pET5a_rhMMP-2 clone. Quantification of MMP-2 was performed using a BCA kit assay. The MMP-2 activity and purity assessments were determined by gel zymography and silver staining. C57BL / 6 [Wild type (Wt)] male mice young adults (7 weeks) (CEUA n° 6175230518), with 20-25g, separated into two groups. MMP-2 group was treated with rhMMP-2 (150 ng/g/i.p);), and the control group was treated with control (0.9% saline solution). Elastin and collagen contents were evaluated using hematoxylin/eosin, orcein, and Picro Sirius red-stain sections. Results: Recombinant MMP-2 administration for four weeks did not change the thickness of the aortic medial layer, the elastin, and collagen content compared to the control animals. Conclusion: No morphological changes were observed in this study. Thus, we will carry out biochemical measurements to verify changes in the aortas' oxidative system due to the increase in MMP-2 levels.

• Palavras-chave
• Fibrosis; aorta remodeling; hypertrophy
• Modalidade
• Vídeos
• Área Temática
• Inflamação e doenças vasculares