Evaluation of the Effects of a Sucrose-Rich Diet on the Development of Diabetic Cardiomyopathy

Evaluation of the Effects of a Sucrose-Rich Diet on the Development of Diabetic Cardiomyopathy

• Autor
• João Paulo Nascimento Miranda
• Co-autores
• Antônio Marcus de Andrade Paes , Gilberto de Holanda Lopes Filho , Mariana Uchoa , Perla Freitas Lopes , Lucas Martins França
• Resumo

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  Introduction: The DOHAD concept study the importance of stressors early in life for the long-term programming of human metabolism. Between these, the food energy imbalance, mainly with the high consumption of sucrose, has led to metabolic syndrome and diabetic cardiomyopathy, mainly by the glucose-insulin axis disorder that can leads to morphofunctional changes. Protein acetylation is one of the most investigated epigenetic mechanisms related to the DOHAD concept. Thus, the study aims to evaluate the effects of a diet rich in sucrose in animal hearts and your protein acetylation. Methods: 14 male wistar rats (CEUA - code 115.016318/2016-96) were divided into 2 groups: Control (CTR=6) and high sugar diet (HSD=8) and fed for 150 days with metabolic assessments and, after euthanasia, removal of samples for Western Blot . In other group (CEUA - register number 23115.007690/2016-10), 16 animals: (CTR=8) and high sugar diet (HSD=8) underwent the same process of induction and evaluation, but with samples after euthanasia for histology. Results: The fisrt group had systemic metabolic disorder, characterized by hypertriglyceridemia (CTR vs. HSD=81,8±8,608 vs. 192,1±20,86mg/dl), alteration of the glucose-insulin axis like ITT (CTR vs HSD=4361±181,1 vs. 5198±170,7mg/dl) and GTT (CTR vs. HSD=14073±190,6 vs. 17067±852,5mg/dL) and others. In evaluation of protein acetylation, a significant increase was found (CTR vs. HSD=0,2744±0,0588 vs. 0,5281±0,0768). In second group of animals, next data were found for metabolic changes, suggesting that the groups are similar. Thus, histological changes of the heart were investigated and founded with an increase in cardiomyocyte hypertrophy (HSD=767987±49121 vs. CTR=570501±29097; p=0,0043) and perivascular fibrosis (HSD=3,174±0,4327 vs. CTR=1,442±0,2473; p=0,0042). Conclusion: We conclude that the sucrose-rich diet is responsible for diabetic cardiomyopathy characterized by histological changes found early in thsi study. Finally, we suggest that the diet is related to increased protein acetylation and is associated with morphofunctional changes consistent with diabetic cardiomyopathy.

• Palavras-chave
• Protein acetylation, Diabetic cardiomyopathy, metabolic syndrome.
• Modalidade
• Vídeos
• Área Temática
• Sinalização celular