Decreased Activity of Extracellular Matrix Metalloproteinases (MMPs) by the Aqueous Extract of Coriandrum sativum L. (CSAE) Prevents Vascular Remodeling in the Progeny of Rats Exposed to Methylmercury During Gestational and Lactational Periods

Decreased Activity of Extracellular Matrix Metalloproteinases (MMPs) by the Aqueous Extract of Coriandrum sativum L. (CSAE) Prevents Vascular Remodeling in the Progeny of Rats Exposed to Methylmercury During Gestational and Lactational Periods

• Autor
• Keuri Eleutério Rodrigues
• Co-autores
• Wictória Farias Dias , Pricila Rodrigues Goncalves , Stefanne de Cássia Pereira da Silva , Thayná Matos da Cunha Catete , David Silva da Costa , Cristine Bastos do Amarante , Raquel Fernanda Gerlach , Moisés Hamoy , Alejandro Ferraz do Prado
• Resumo

• Mercury-induced toxicity promotes impairment of the cardiovascular system, which may be related to changes in extracellular matrix proteins (MMP) activity, proteases involved in cardiovascular remodeling. Screening new compounds that decrease toxicity induced by mercury, Coriandrum sativum showed antioxidant activity and capacity to detoxifies mercury compounds. Thus, the present study aimed to evaluate the effects of aqueous extract of C. sativum (CSAE) on mercury levels, oxidative stress, vascular remodeling, and MMP activity in the aorta of the progeny of rats exposed to methylmercury (MeHg). The animals were randomized into 4 groups (n: 4) Control; CSAE; MeHg; MeHg + CSAE. Rats were intoxicated in the 14th gestational day with MeHg in drinking water (40 µg/mL) and co-treated with CSAE (360 mg/kg per day) until the 14th day of lactation (21 days of treatment) (CEUA nº 3610090519). Oxidative stress was measured by the DPPH test, DHE probe, and lipid peroxidation. Aortic morphometry, elastin, and collagen contents were evaluated using hematoxylin/eosin, orcein, and Picro Sirius red-stain sections. Finally, the level of mercury in the hair was assessed. The CSAE inhibited the DPPH radical. In our results. The MeHg Group showed higher levels of mercury compared to the control and MeHg + CSAE. The MeHg group showed a decrease in the middle layer's thickness, with a reduction in the elastin and collagen contents compared to the control and MeHg+CSAE. The MeHg group showed an increase in gelatinolytic activity compared to the control and MeHg + CSAE. The MeHg group showed higher levels of reactive oxygen species and lipid peroxidation than the control and MeHg + CSAE. In conclusion, the CSAE prevented aortic remodeling and MMP activity by reducing oxidative stress and MeHg levels.

• Palavras-chave
• Remodeling, Atrophy, Toxicology
• Modalidade
• Vídeos
• Área Temática
• Produtos naturais