INTRODUCTION: Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome, are inflammatory diseases with high mortality and morbidity rates. However, treatments are non-specific, making it crucial to search for therapeutic alternatives [1]. Studies have revealed several pharmacological properties of species of the genus Miconia, including anti-inflammatory activity [2]. AIMS: Therefore, the study sought to evaluate the anti-inflammatory activity of ethyl acetate extract from the aerial parts of Miconia serialis (MsAcOEt) in an in vivo model of Lipopolysaccharide (LPS)-induced ALI. METHODS: MsAcOEt was obtained by exhaustive maceration and a qualitative phytochemical screening was carried out. The ALI model was carried out with groups of Balb/c mice, which received oral pre-treatment with saline solution (NaCl 0.9%), dexamethasone (1 mg/kg) or MsAcOEt in the doses of 50, 100 and 200 mg/kg, 1 hour before intranasal stimulation with LPS [3]. After 24 hours, euthanasia was performed by anesthetic overdose and bronchoalveolar lavage fluid (BALF) was collected to analyze leukocyte migration, with total and differential counts. Lung tissue was removed to determine NO levels, using the Griess reaction, and to perform histological analysis [4]. Statistical analysis was performed by one-way ANOVA, followed by Dunnett's test. The work was approved by the ethics committee of the Federal University of Pernambuco (No. 0057/2022). RESULTS AND DISCUSSION: MsAcOEt presented alkaloids, triterpenes, steroids and flavonoids, which are associated with biological activities in the literature [5]. In the ALI model, MsAcOEt inhibited leukocyte migration in BALF with rates of 63.8%, 85.2% and 93.2% at doses of 50, 100 and 200 mg/kg, respectively. These doses also inhibited the recruitment of neutrophils, monocytes and eosinophils, with rates between 50% and 95.1%, thus promoting an anti-inflammatory effect, as they are important cells for the inflammatory characterization of ALI [6,7]. Lymphocytes, on the other hand, were inhibited by 78.6% only at a dose of 100 mg/kg, but they are not relevant in the acute phase of ALI [8]. During inflammation, NO is found at high levels, promoting oxidative and tissue damage [9]. In this study, NO was reduced at doses of 50 (4.63 ± 0.25 mM), 100 (1.70 ± 0.21 mM) and 200 mg/kg (1.07 ± 0.15 mM) compared to injured control (6.05 ± 0.60 mM), thus indicating an attenuation of this damage. Histological analysis revealed that the pre-treated groups demonstrated a reduction of damage characteristics of ALI, presenting preserved bronchioles and few regions with cellular infiltrates and thickening of the alveolar septa. CONCLUSION: The study proves the anti-inflammatory activity of M. serialis extract, which could be associated with the presence of compounds detected in this extract. ACKNOWLEDGEMENT: We appreciate the contribution of the members of the Laboratório de Prospecção Farmacotoxicológica de Produtos Bioativos.
Keywords: Gênero Miconia, Modelo in vivo, Inflamação.
REFERENCES
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Comissão Organizadora
Francisco Mendonça Junior
Pascal Marchand
Teresinha Gonçalves da Silva
Isabelle Orliac-Garnier
Gerd Bruno da Rocha
Comissão Científica
Ricardo Olimpio de Moura
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