CHEMICAL PROFILE AND ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT FROM LEAVES AND SEEDS OF Anadenanthera Colubrina (Vell.) Brenan

CHEMICAL PROFILE AND ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT FROM LEAVES AND SEEDS OF Anadenanthera Colubrina (Vell.) Brenan

• Author
• MARIA INÁCIO DA SILVA
• Co-authors
• Mariana Pereira da Silva , José Jonas Ferreira Viturino , Joice Barbosa do Nascimento , Ana Cecília Calixto Donelardy , Geane Gabriele de Oliveira Souza , José Walber Gonçalves Castro , José Galberto Martins da Costa
• Abstract

•  

  INTRODUCTION: It is essential to carry out studies aimed at antioxidant evaluation of caatinga plants. The species Anadenanthera Colubrina (Vell.) Brenan (Fabaceae), popularly known as angico-branco, is considered a plant with therapeutic potential. It is popularly used to treat respiratory and skin problems and inflammation in general [1]. AIMS: Identify classes of secondary metabolites in the leaves and seeds of A. colubrina ethyl acetate extract, and evaluate the antioxidant activity through different mechanisms of action. METHODS: The leaves and seeds of this species were collected in Juazeiro do Norte, Ceará, Brazil. The extracts were prepared by soxhlet extraction for 4h, using ethyl acetate as solvent and concentrated in a rotary evaporator. Chemical prospecting occurred through qualitative coloring and precipitation reactions [2]. To carry out the antioxidant tests, both extracts were diluted in concentrations ranging from 50 to 1000 µg/mL. After the samples' incubation period, absorbance measurements were determined using the photocolorimetric method on a UV-visible spectrophotometer at 518 nm for DPPH and 734 nm for ABTS [3]. The data obtained were processed using the Graph Prism® version 8.1 program. RESULTS AND DISCUSSION: In the chemical evaluation it was possible to identify the presence of condensed tannins, flavones, flavonols, xanthones, chalcones, aurones and catechins. The DPPH concentrations of the extracts showed IC50 71.36 in the leaves and 70.66 in the seeds. In the ABTS assay, the IC50 was 284.7 and 190.9 respectively. These results presented a level of significance (p <0.05) when compared between the different extracts tested. CONCLUSION: Both extracts showed antioxidant potential, which varied according to the different concentrations tested. It is pertinent to continue developing experimental studies to identify and correlate the compounds responsible for carrying out these activities and their therapeutic application. ACKNOWLEDGEMENT: Natural Products Laboratory of the Regional University of Cariri and Ceará Foundation for Support to Scientific and Technological Development.

  REFERENCES

  [1] JUNIOR, O. C, Lima NM, Silva MGA, Aguiar VB, Carli GP, Scherrer EC, Castro SBR, Alves CCS, Oliveira MAL, Carli AP. In vitro and in vivo evaluation of anti-inflammatory activity and free radical scavenging potential of leaves extract from Anadenanthera colubrina. Nat Prod Res Nov;35(22):4819-4823, 2021.

  [2] MATOS, F. J. A. Introduction to experimental phytochemistry, 3rd. ed. Fortaleza: UFC, 2009.

  [3] RUFINO, M. S. M., ALVES, R. E., BRITO, E. S., MORAIS, S. M., SAMPAIO, C.G., JIMÉNEZ, J. P., CALIXTO, F.D.S. (2007). Scientific methodology: determination of total antioxidant activity in fruits by capturing the free radical DPPH and ABTS. Embrapa ISSN 1679- 6535.

   

• Keywords
• Anadenanthera colubrina, chemical prospecting, , antioxidant activity.
• Modality
• Pôster
• Subject Area
• Drug Design and Discovery, Synthesis and Natural Products