CHEMICAL PROFILE AND ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACT FROM THE STEM BARK OF Cnidoscolus quercifolius Pohl.

CHEMICAL PROFILE AND ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACT FROM THE STEM BARK OF Cnidoscolus quercifolius Pohl.

• Author
• Joice Barbosa do Nascimento
• Co-authors
• José Jonas Ferreira Viturino , Maria Inácio da Silva , Mariana Pereira da Silva , Ana Cecília Calixto Donelardy , José Walber Gonçalves Castro , Fabiola Fernandes Galvão Rodrigues , José Galberto Martins da Costa
• Abstract

•  

  INTRODUCTION: The species Cnidoscolus quercifolius (Euphorbiaceae), endemic to the Caatinga, is popularly used in traditional medicine in the semi-arid region, however, there are few reports in the literature proving its therapeutic potential [1]. AIMS: Trace the chemical profile of the methanolic extract (EMCq) of the bark of the stem of C. quercifolius, as well as evaluate its antibacterial and modulating activity of the antibiotic activity of aminoglycosides against strains of multi-resistant bacteria. METHODS: The extract was obtained using a Soxhlet system and the identificatin of classes of secondary metabolites was carried out through qualitative chemical prospecting. In antibacterial tests, multi-resistant bacterial strains of S. aureus (SA 10), E. coli (EC 06), K. pneumoniae (BLSE) and K. pneumoniae (KPC) were used, and using the microdilution method, the Minimum Inhibitory Concentration (MIC) of each strain was determined and the modulating potential of the antibiotic activity of aminoglycosides was evaluated. RESULTS AND DISCUSSION: The chemical test identified the presence of the following classes of metabolites: flavones, flavonols, xanthones, flavonones, flavononols, catechins, leucoanthocyanidins and alkaloids, however, the presence of tannins was not observed. EMCq demonstrated a clinically useful antibacterial effect against BLSE and KPC with MICs of 256 and 512 ?g/mL, respectively. Furthermore, the extract in association with amikacin showed synergism against all strains under study, when combined with gentamicin, the extract potentiated the action of the drug against SA 10, EC 06 and BLSE, on the other hand, there was equivalence of means when tested against KPC. These results indicate that the substances tested increase the effectiveness of these drugs, which may be related to interference in the resistance mechanism of microorganisms, as bioactive compounds present in plant extracts, such as flavonoids and alkaloids, are capable of hindering the development of mechanisms defense against these pathogens [2]. CONCLUSION: The stem bark of C. quercifolius has bioactive compounds with the potential to be used as an alternative to combat multi-resistant bacteria, however, more specific investigations are still necessary to elucidate its mechanism of action on these microorganisms. ACKNOWLEDGEMENT: Coordination for the Improvement of Higher Education Personnel.

  REFERENCES

  [1] RIBEIRO, P. P. C., MEDEIROS, J. M. S., & DA SILVA CHAVES, K. S. F. Cnidoscolus quercifolius: Nutritional value, bioactive activity and potential application of seed and its derivatives in human nutrition. Trends in Food Science & Technology, 105, 70-75, 2020.

  [2] CAMILO, C. J., NONATO, C. D. F. A., DA SILVA, J. T. P., RODRIGUES, F. F. G., SALAZAR, G. J. T., & DA COSTA, J. G. M. Interferência do Extrato de Hymenaea courbaril L. (Jatobá) na Atividade Antibacteriana de Aminoglicosídeos. Revista Interfaces: Saúde, Humanas e Tecnologia, v. 8, n. 1, p. 372-379, 2020.

• Keywords
• Cnidoscolus quercifolius, antibacterial activity, chemical composition.
• Modality
• Pôster
• Subject Area
• Drug Design and Discovery, Synthesis and Natural Products