EVALUATION OF THE ANTIOXIDANT ACTIVITY AND CHEMICAL PROFILE OF DYSIDEA ETHERIA AND DESMAPSAMMA ANCHORATA EXTRACTS

EVALUATION OF THE ANTIOXIDANT ACTIVITY AND CHEMICAL PROFILE OF DYSIDEA ETHERIA AND DESMAPSAMMA ANCHORATA EXTRACTS

• Author
• Geane Gabriele de Oliveira Souza
• Co-authors
• José Walber Gonçalves Castro , Ana Cecília Calixto Donelardy , Alexandro Rodrigues Dantas , Carla de Fatima Alves Nonato , Johnatan Wellisson da Silva Mendes , Cicera Janaine Camilo , José Galberto Martins da Costa
• Abstract

• INTRODUCTION: Research with species of marine sponges has sparked interest in research with natural products. These species have various substances in their composition that may be associated with activities such as antioxidant, antimicrobial, anticancer, among others. As a result, investigations into the chemical composition and antioxidant profile are of great importance in identifying new active ingredients [1]. AIMS: Qualitatively identify the chemical classes present in the methanolic extract of the species Dysidea etheria and Desmapsamma anchorata and determine the antioxidant activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH•) stable free radical scavenging method. METHODS: The qualitative chemical profile was evaluated by adding different reagents, observing whether there was a variation in the color of the solution [3]. The antioxidant activity was determined by the DPPH free radical scavenging method at different sample concentrations [2]. RESULTS AND DISCUSSION: Chemical analysis showed the presence of flavones, flavonols, xanthones, chalcones, aurones, catechins and leocoanthocyanidins in both samples. For antioxidant activity, it was demonstrated that both extracts did not present antioxidant activity at the concentrations tested, since the values obtained in this assay did not make it possible to calculate the IC50, in which the positive control of ascorbic acid presented an IC50 of 17.55µg/ mL. CONCLUSION: Marine sponges are promising natural sources for extracting bioactive compounds. Although the values obtained are not significant, regarding the DPPH method, it is necessary to carry out new research addressing other methods, since chemical compounds react in different ways depending on the mechanism of action. ACKNOWLEDGEMENT: FUNCAP and LPPN URCA.

   

  REFERENCES

  [1] VARIJAKZHAN, Disha. et. al. Bioactive Compounds from Marine Sponges: Fundamentals and Applications. 5. ed. Malaysia: Editora Marine drugs, 2021.

  [2] RUFINO, M. S.; ALVES, R. E.; BRITO, E. S.; MORAIS, S. M.; SAMPAIO, C. G.; PÉREZJIMÉNEZ, J.; SAURA-CALIXTO, F. D. Scientific Methodology: Determination of Total Antioxidant Activity in Fruits by Capture of DPPH Free Radical. Fortaleza: Embrapa, 2007.

  [3]MATOS, F. J. A. Introduction to experimental phytochemistry, 3rd, ed. Fortaleza: UFC, 2009.

   

   

• Keywords
• Antioxidant, Marine sponges, DPPH.
• Modality
• Pôster
• Subject Area
• Drug Design and Discovery, Synthesis and Natural Products