ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF THE HYDROALCOHOLIC EXTRACT OF THE LEAVES OF Terminalia catappa L. (COMBRETACEAE)

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF THE HYDROALCOHOLIC EXTRACT OF THE LEAVES OF Terminalia catappa L. (COMBRETACEAE)

• Author
• Pedro Victor Rodrigues da Silva
• Co-authors
• Alexandra Débora Leite Borba , Elizabeth Fernanda de Oliveira Borba , Natacha Ferreira Albino Paixão , Yasmim Meneses Silva , Beatriz de Fátima Maia de Santana , Abner Lins Dantas , Teresinha Gonçalves da Silva
• Abstract

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  ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF THE HYDROALCOHOLIC EXTRACT OF THE LEAVES OF Terminalia catappa L. (COMBRETACEAE)

  Pedro Victor Rodrigues da Silva ¹; Alexandra Débora Leite Borba¹; Elizabeth Fernanda de Oliveira Borba¹, Teresinha Gonçalves da Silva^1*

  ¹Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco

   

  pvrfarmacia@gmail.com; aledborba92@gmail.com; elizabethfernanda_7@hotmail.com; teresinha.goncalves@ufpe.br

   

  ABSTRACT

  INTRODUCTION: Terminalia catappa L., a member of the Combretaceae family, is commonly known as “mendoeira”, “amendoeira-da-praia” ou “sombreiro” [1]. It plays an important role in folk medicine, being an alternative in the treatment of infections, inflammations and other health problems [2]. In addition, its fruits and seeds are used as food in various communities [3]. AIMS: To analyze the phytochemical profile and assess the antioxidant and antimicrobial activities of the hydroalcoholic extract of Terminalia catappa leaves (EHTc). METHODS: The phytochemical profile of the extract was analyzed using thin-layer chromatography (DLC) [4] and the compounds were identified using ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-DAD-QTOF MS). The levels of total phenols, total tannins [5] and flavonoids [6] were also quantified. The antioxidant potential was assessed using the DPPH, ABTS and phosphomolybdenum tests [7]. Cytotoxicity [8] was tested against red blood cell, fibroblast, macrophage and keratinocyte strains, while antimicrobial activity was determined using broth microdilution tests and biofilm inhibition and removal tests [9]. RESULTS AND DISCUSSION: The types of secondary metabolites detected through UPLC-DAD-QTOF MS analysis were flavonoids, hydrolyzable tannins, alkaloids, triterpenes and steroids, which are in line with the findings of Krishnaveni et al. (2015). The EHTc revealed levels of total phenols (3,79 g/EAG/g), total tannins (2,16 g/EAT/g) and flavonoids (2,34 g/EQ/g), as well as antioxidant activity in the different methods analyzed. EHTc did not present toxicity towards L929 cells (murine fibroblasts) and murine peritoneal macrophages and did not cause hemolysis at the concentrations tested. In the broth microdilution assay, EHTc showed moderate inhibition against the bacteria Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae and Proteus mirabilis, and against the fungus Candida albicans, confirmig this with data fround by Wen and collaborators (2011). Biofilms of Candida spp. they showed low metabolic activity when in contact with EHTc at concentrations equal to or greater than 124 ?g/mL, being more effective in inhibiting the formation of Candida tropicalis and Candida parapsilosis biofilms. CONCLUSION: The study showed that EHTc has remarkable antioxidant and antimicrobial potential, while demonstrating low cytotoxicity. These data suggest a possible association between these properties and the presence of phenolic compounds.

  ACKNOWLEDGEMENT: The authors are grateful the “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES) for granting the research scholarship.

   

  Keywords: Extract; Phytochemistry; Activity.

   

   

  REFERENCES

  [1] IVANI, Silvia de Azevedo et al. Morfologia de frutos, sementes e plântulas de castanheira (Terminalia catappa L.-combretaceae). Revista Brasileira de Fruticultura, v. 30, p. 517-522, 2008.

   

  [2] KANERIA, Mital J. et al. Nontargeted metabolomics approach to determine metabolites profile and antioxidant study of Tropical Almond (Terminalia catappa L.) fruit peels using GC-QTOF-MS and LC-QTOF-MS. Journal of Pharmaceutical and Biomedical Analysis, v. 160, p. 415-427, 2018.

   

  [3] AGU, Chinedu Matthew et al. Modeling of methyl ester yield from Terminalia catappa L. kernel oil by artificial neural network and response surface methodology for possible industrial application. Cleaner Engineering and Technology, v. 6, p. 100360, 2022.

   

  [4] WAGNER, H; BLADT, S. Plant drug analysis: a thin layer chromatography atlas. 2 a edição. Nova York: Springer, 2009.

   

  [5] LI, C. Y. et al. Gastroprotective effect of cyanidin 3-glucoside on ethanol-induced gastric lesions in rats. Alcohol, v.40, n.8, p.683-687, 2008.

   

  [6] WOISKY, R.G.; SALATINO, A. Analysis os propolis: some parameters ond prodecore for chemical fuality control. Journal of Apicultural Research, v.37, n.2, p.99-105, 1998.

   

  [7]VERAS, B.O. et al. Chemical composition and evaluation of the antinociceptive, antioxidant and antimicrobial effects of essential oil from Hymenaea cangaceira (Pinto, Mansano & Azevedo) native to Brazil: A natural medicine. Journal of Ethnopharmacology, v.247, n. 112265, p.1-9, 2020.

   

   [8]ALLEY, M. C. et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Research, v. 48, n. 3, p. 589- 601, fev.1988.

   

  [9]CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 28th. ed. Wayne, Pennsylvania: Clinical and Laboratory Standards Institute, 2018. 

   

  [10] KRISHNAVENI, M. et al. Gas chromatography-mass spectrometry/mass spectrometry analysis of Terminalia catappa l. nut and antimicrobial assay. Asian Journal of Pharmaceutical and Clinical Research , v.8, n.4, p.168-170, 2015.

   

  [11] WEN, K.C. et al. Inhibitory Effects of Terminalia catappa on UVB-Induced Photodamage in Fibroblast Cell Line. Evidence-Based Complementary and Alternative Medicine, p.1-9, 2011.

   

   

   

• Keywords
• Extract; Phytochemistry; Activity.
• Modality
• Pôster
• Subject Area
• Drug Design and Discovery, Synthesis and Natural Products