EVALUATION OF ANTIOXIDANT ACTIVITY AND QUANTIFICATION OF TOTAL PHENOLS IN THE ETHANOLIC EXTRACT OF GARCINIA BRASILIENSIS LEAVES

EVALUATION OF ANTIOXIDANT ACTIVITY AND QUANTIFICATION OF TOTAL PHENOLS IN THE ETHANOLIC EXTRACT OF GARCINIA BRASILIENSIS LEAVES

• Author
• Alexandro Rodrigues Dantas
• Co-authors
• Mariana Pereira da Silva , Johnatan Wellisson da Silva Mendes , Geane Gabriele de Oliveira Souza , José Walber Gonçalves Castro , Fabíola Fernandes Galvão Rodrigues , Lariza Leisla Leandro Nascimento , José Galberto Martins da Costa
• Abstract

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  INTRODUCTION: The genus Garcinia has been shown by phytochemical studies to have a great diversity of bioactive compounds, such as benzophenones, xanthones and flavonoids, such as 7-Epiclusianone and moreloflavone [1]. Many of these constituents are active ingredients with pharmacological properties against various diseases [2]. Garcinia brasiliensis Mart. Also known as bacupari, bacopari, bacupari-miúdo or yellow mangosteen, it has fruits appreciated both in fresh consumption and their derivatives. Research points to the immunotoxic, anti-inflammatory, antioxidant and anticancer potential of biflavonoid substances and benzophenones extracted from bacupari leaves and fruits [3]. AIMS: Analyze the total phenolic content of the ethanolic extract of Garcinia brasiliensis leaves and evaluate the antioxidant activity. METHODS: The ethanolic extract of Garcinia brasiliensis leaves (EEFGB) (525 kg) was obtained by maceration in ethanol for 72h, then the solvent was distilled on a rotary evaporator, obtaining a yield of 5.78%. The quantification of total phenols followed the Folin-Ciocalteu oxidation method, using gallic acid as standard, and results were expressed in mg gallic acid equivalent per g of extract (mg GA/g Ext.). Antioxidant activity was evaluated using the DPPH• free radical scavenging and ABTS+ free radical scavenging assays, both at different concentrations (ranging from 5.0 to 1000 µg/mL). The tests were read using an Elisa and UV-visible spectrophotometer at 518 nm and 734 nm, respectively, using ascorbic acid as a standard for both tests. RESULTS AND DISCUSSION: In the quantification of total phenols, the presence of 253 mg GA/g Ext was verified. For antioxidant activity, EEFGB presented significant values in the elimination of the DPPH• radical, with IC50 of 117.0. Studies show that as a defense mechanism, plants can increase phenolic production in response to oxidative pressure and excess radiation [4], these secondary metabolites preserve their antioxidant properties in human organisms [5]. They have been investigated for therapeutic purposes. CONCLUSION: Therefore, EEFGB has a significant amount of phenols and has antioxidant potential relevant to the DPPH• and ABTS+ methods. Additional research is needed on antioxidant action through different reaction mechanisms. ACKNOWLEDGEMENT: Coordination for the Improvement of Higher Education Personnel (CAPES), Regional University of Cariri (URCA), Natural Products Research Laboratory (LPPN).

   

• Keywords
• Antioxidant, Natural products, Garcinia brasiliensis
• Modality
• Pôster
• Subject Area
• Drug Design and Discovery, Synthesis and Natural Products