PHYTOCHEMICAL SREENING AND IN VIVO WOUND HEALING ACTIVITY OF Micononia minutiflora (Bonpl.) DC. (MELASTOMATACEAE)

PHYTOCHEMICAL SREENING AND IN VIVO WOUND HEALING ACTIVITY OF Micononia minutiflora (Bonpl.) DC. (MELASTOMATACEAE)

• Author
• Maria Luísa Novaes Cahú
• Co-authors
• Aline Stamford Henrique da Silva Guerra Gatis Carrazzoni , José Rivaldo de Lima , Teresinha Gonçalves da Silva , Abner Lins Dantas , Tonny Cley Campos Leite , Elizabeth Fernanda de Oliveira Borba , Stella de Jesus Lourenço da Silva
• Abstract

• INTRODUCTION: Miconia is the largest genus in the Melastomataceae family, predominantly found in South and Central America [1]. The popular uses of specimens of this genus are increasingly evident in treating inflammation, arthritis, rheumatism and colds [2, 3]. On the one hand, several biological activities of Miconia species have already been described, including antioxidant, anti-inflammatory, anti-hyperalgesic properties, among others [4, 5]. AIMS: In this work, the objective was to evaluate the healing activity of the semi-solid formulation of the methanolic extract of M. minutiflora leaves (Mm-meOH). METHODS: For this, the leaves of M. minutiflora were collected in the Rio Largo region, in Alagoas State and an exsicata was deposited in the IMA-AL Herbarium under number 50397. The extract was obtained by the maceration method, using methanol as solvent. Subsequently, a phytochemical screening of the extract was carried out by Thin Layer Chromatography (TLC). Three groups were used: G1- the negative control (lanovaselin ointment); G2- treated with dexpanthenol (50 mg/kg); and G3- treated with the semi-solid formulation containing Mm-MeOH (FSSMm 10%). The groups were subdivided into three categories, considering the observation period (3, 7 and 14 days). The wound areas were photographed at different times after surgery and tissue samples were collected and subjected to histopathological analysis. RESULTS AND DISCUSSION: The phytochemical profile by TLC revealed the presence of alkaloids, phenolic compounds, mono-, sesqui- and diterpenes, triterpenes, steroids and anthracene derivatives. In terms of healing activity, the group treated with FSSMm 10% reduced the injured area after 7 and 14 days (79.2 ± 3.6% and 90.13 ± 4.1%, respectively) in relation to the negative control. In the histopathological evaluation, after 3 days of treatment with 10% FSSMm, a proliferation of adipocytes and fibroblasts, connective tissue with collagen and the beginning of the appearance of granulation tissue were observed. After 7 days of treatment, adipose tissue, fibroplasia and epidermis formation were observed. After 14 days, closure of the lesion, absence of epidermal hyperplasia, presence of collagen bundles, deep adipose tissue and newly formed skin appendages were observed. CONCLUSION: According to the results, the Mm-MeOH extract showed advancement in the healing process evidenced by the reduction of exudation, edema and hyperemia in the injured area. These effects can be attributed to the presence of secondary metabolites present in the extracts, notably phenolic compounds. However, purified fractions must be tested to confirm the metabolites responsible for this effect. ACKNOWLEDGEMENT: We thank the Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE) for the Support.

• Keywords
• methanolic extract, medicinal plants, wounds.
• Modality
• Pôster
• Subject Area
• Drug Design and Discovery, Synthesis and Natural Products